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Том 60, № 10 (2024)

Мұқаба

Бүкіл шығарылым

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

ОБЗОРНЫЕ И ТЕОРЕТИЧЕСКИЕ СТАТЬИ

Epigenetic Mechanisms of the Influence of Physical Activity on the Development of Atherosclerosis

Mustafin R., Khusnutdinova E.

Аннотация

This work is an analytical review dedicated to the search for driver mechanisms of epigenetic changes in atherosclerosis pathogenesis. The disease affects the cardiovascular system in the adult population, mainly the elderly and senile. Atherosclerosis is accompanied by progressive deposition of cholesterol and lipoproteins in vessels intima with inflammation, narrowing of the lumen and impaired blood supply to tissues and organs. These processes are characterized by changes in the expression of CACNA1C, GABBR2, TCF7L2, DCK, NRP1, PBX1, FANCC, CCDC88C, TCF12, ABLIM1 genes. Prevention of atherosclerosis is physical activity, the mechanisms of which are not fully understood. Experimental models have shown that regular training not only has a protective effect on the development of atherosclerosis, but also inhibits the progression of an already developed disease with a decrease in vascular stenosis, an increase in the concentration of collagen and elastin and matrix metalloproteinases in plaques. These results have been confirmed by clinical studies. The purpose of this review was to systematize the accumulated results on the causes of epigenetic changes, including those under the influence of regular training, causing changes in the expression of specific microRNAs in atherosclerosis. It was found that physical exercise in Apo-/- mice increases the expression of miR-126 and miR-146a (inhibiting the TLR4 and TRAF genes), miR-20a (affecting PTEN), miR-492 (suppressing RETN gene mRNA). Clinical studies have shown an increase in the levels of miR-146a, miR-126, miR-142-5p, miR-424-5p and a decrease in the transcription of miR-15a-5p, miR-93-5p, miR-451 under the influence of aerobic training. It has been suggested that the drivers of epigenetic changes in atherosclerosis are transposons pathologically activated during aging, the transcription of which can change under the influence of physical training, which is accompanied by impaired expression of long non-coding RNAs and microRNAs derived from transposons. Analysis of the literature data allowed us to identify 36 such microRNAs, 25 of which showed identical changes in levels during aging and atherosclerosis.

Genetika. 2024;60(10):3-21
pages 3-21 views

The Meiotic Drive: Intragenomic Competition and Selection

Zakharov I.

Аннотация

The article considers the distribution and mechanisms of the meiotic drive as a phenomenon manifested in unequal transmission of gene alleles and/or homologous chromosomes into gametes during meiosis. The meiotic drive has been studied in the most detail in Drosophila, mice, corn and in ascomycete fungi of the genera Neurospora and Podospora. The consequence of the meiotic drive is a shift in the frequencies of alleles in the gene pool and the maintenance of non-adaptive traits in the population.

Genetika. 2024;60(10):22-30
pages 22-30 views

ГЕНЕТИКА РАСТЕНИЙ

Genome-Wide Identification and Characterization of Sugar Transporter Genes in Silver Birch

Korzhenevskyi M., Moshchenskaya Y., Tarelkina T., Galibina N.

Аннотация

Sugar transporters play an important role in regulating the long-distance sucrose transport from source to sink organs. The main sucrose absorber in woody plants is developing wood. Thus, sucrose transport, regulated by SUT, SWEET, and MST gene families, will determine the formation of woody biomass. Based on silver birch (Betula pendula var. pendula Roth) genomic data, we identified and analyze encoding sugar transporters in Betula pendula. We conducted BLAST-search, phylogenetic, structural analysis and analysis of cis-acting elements of sugar transporter genes and determined their chromosomal localization. We were able to identify and characterize 3 genes of the SUT family, 10 SWEET genes and 36 MST genes, which have a typical number of functional and transmembrane domains for the family. It was shown that silver birch contains a smaller number of sugar transporters genes compared to A. thaliana, which is probably because of the apoplastic type of terminal phloem loading in Arabidopsis, while in silver birch phloem loading is carried out predominantly symplastically. The results obtained may be useful for further study of the participation of sucrose transporters in various biosynthetic processes in woody plants and provide a basis for various biotechnological manipulations.

Genetika. 2024;60(10):31-46
pages 31-46 views

Comparative Analysis of Mutations of the Gynogenesis Maize Genes

Moiseeva Y., Fadeev V., Fadeeva Y., Mazilov S., Chumakov M.

Аннотация

The SNP polymorphism in gynogenesis (Zm_Pla1, Zm_CenH3, Zm_Dmp7) genes from haploid-inducing and control maize lines was evaluated. Using sequencing and multiple alignment methods the SNP, deletions, insertions in the target genes was established in comparing with the reference B73 maize line, and the phylogenetic trees were prepared. The presence of a 4-nucleotide insertions (4NI) and 15 identical SNP in the Zm_Pla1 gene of the ancestral Stock 6 and haploid-inducing ZMS-8 and ZMS-P maize lines and the absence of 4NI in the Zm_Pla1 gene in parthenogenetic as well as control maize lines were established. Phylogenetic analysis of the Zm_Pla1 gene confirmed the relationships between Stock 6 and haploid-inducing ZMS-P and ZMS-8 maize lines from Saratov. The presence of 5 SNPs in the Zm_Dmp7 gene of the ZMS-8 line, 3 SNPs and one 3-nucleotide deletion in the Zm_Dmp7 gene of the ZMS-P line, 3 SNPs and a 9-nucleotide deletion in the Zm_Dmp7 gene of the KM line were observed. The SNP (at position 131 from the start codon) in the Zm_Dmp7 gene is the reason for the increased haploid-inducing ability in CAU5, but not for ZMS-P maize line. Additionally, the 3-nucleotide deletion in the Zm_Dmp7 gene in the ZMS-P maize line and a 9-nucleotide deletion in the KM maize line were observed.

Genetika. 2024;60(10):47-55
pages 47-55 views

Cysteine-Rich Peptide Genes of Wheatgrass Thinopyrum elongatum

Slezina M., Istomina E., Shiyan A., Odintsova T.

Аннотация

Cysteine-rich peptides play an important role in the plant defense system. The aim of the present work was to search in silico for genes encoding antimicrobial and signaling peptides in the genome of Thinopyrum elongatum (Host) D.R. Dewey (2n = 14, EE) − a wild grass species with high resistance to pathogens and abiotic stress. Bioinformatic analysis revealed 154 new genes of antimicrobial and signaling peptide precursors belonging to 9 families in Th. elongatum genome. Introns were detected in a number of cysteine-rich peptide genes. The structure of peptide precursors and localization of peptide genes in wheat chromosomes were determined. The greatest similarity of the sequences of Th. elongatum peptides with homologous peptides of plants of the genera Triticum and Aegilops was shown, which confirms the cytogenetic data on the relatedness of genome E with genome D and similar genomes. The results obtained contribute to the characterization of molecular components of the immune system of Th. elongatum and will serve as a basis for further studies of resistance mechanisms, as well as for scientifically justified practical use of this species as a resistance donor in wheat breeding.

Genetika. 2024;60(10):56-70
pages 56-70 views

ГЕНЕТИКА ЖИВОТНЫХ

Phylogeography Patterns and Population Genetics Polymorphism of Daphnia cristata Sars and D. longiremis Sars (Anomopoda: Daphniidae) in North Eurasia

Zuykova E., Sleptzova L., Andreeva L., Kuchko Y., Bochkarev N., Zakharov E.

Аннотация

The first data on the population genetic structure of the D. cristata Sars, 1862 and D. longiremis Sars, 1862 species (Anomopoda: Daphniidae) from the water bodies of North Eurasia are present. The variability of the 12S gene fragment of the mtDNA for these taxa belonging to subgenus Daphnia (Daphnia) O.F. Müller, 1776 (Crustacea: Cladocera) in this region is studied, and the phylogenetic analysis performed. The genealogical relationships between haplotypes of the D. cristata and D. longiremis species and their geographical distribution are shown. Our new data allow us to suggest a recent rapid spatial expansion for D. cristata during the Late Pleistocene and Early-Middle Holocene periods. According to the results of the study, the distribution zones of D. cristata and D. longiremis species are separated in the latitudinal direction with the zone of their contact in Central Yakutia. Our results provide convincing evidence for the influence of ice ages and interglacial on the formation of modern genetic diversity and the phylogeographic structure of D. cristata and D. longiremis.

Genetika. 2024;60(10):71-82
pages 71-82 views

ГЕНЕТИКА ЧЕЛОВЕКА

Uneven Influx of European-Specific Alleles of SLC45A2, SLC24A5, TYRP1, DRD2, EDAR and OCA2 Genes into the Gene Pool of the Koryaks

Malyarchuk B., Litvinov A.

Аннотация

The distribution of alleles highly specific to Europeans in the Koryak gene pool, which formed as a result of intensive interethnic admixture in the Northern Priokhotie, characterized by the prevailing genetic contribution from males of Eastern European origin, was analyzed. The loci rs16891982 (SLC45A2 gene), rs1426654 (SLC24A5 gene), rs1408799 (TYRP1 gene), rs1076563 (DRD2 gene), rs3827760 (EDAR gene), and rs1448485 (OCA2 gene), which are mainly associated with the pigmentation system, were selected for analysis. High heterogeneity was found in the frequency of European-specific alleles, ranging from 1.4% for the variant rs1076563-C of the DRD2 gene to 14.7% for the variant rs1426654-A of the SLC24A5 gene. The reasons for the uneven influx of European-specific alleles into the Koryak gene pool are discussed. It is possible that the formation of genetic structure of modern Koryaks under intensive interethnic admixture was accompanied by the influence of natural selection on some parts of the genome.

Genetika. 2024;60(10):83-89
pages 83-89 views

Dynamics of the Population Structure of the Population of the South of Central Russia Over a 130-Year Period. Age of Marriage

Sergeeva K., Goncharova Y., Nevinnykh A., Batlutskaya I., Sokorev S., Sorokina I.

Аннотация

The article presents the results of assessing the dynamics of the age at marriage of the reproductive part of the population of the south of Central Russia, which acts as one of the factors in the dynamics of the genetic structure of subsequent generations. Over 130 years (from 1890–1910 to 2016–2018), there was an increase in the age of marriage for men (+ 5.11 years, from 24.05 years to 29.16 years) and women (+ 5.78 years, from 20.39 years to 26.17 years) and the average difference in the age of spouses decreased by 0.66 (3.66 to 3.00 years). At the end of the 19th century the marriage age of the urban population of the reproductive part of the population was higher than that of the rural population (especially among men). By the middle of the 19th century the average age of marriage among the rural population increased, exceeding the city indicators and this trend continued in subsequent generations until 2016–2018. There was a decrease in the average age difference between spouses, especially in the urban part of the population. At the end of the 19th century, the average difference in the age of spouses among urban residents was 1.4 times higher than for rural residents, and by the beginning of the 21st century, on the contrary, it was 1.4 times lower. Positive marital assortativeness by age of spouses increased over the 130-year period (1.2 times), both in the city and in the village, but was more significant for residents of rural areas (except for the period 1991–1993). Changes in the average marriage age of spouses that have been occurring in recent decades are of particular significance from a medical-genetic point of view (especially the age at which women marry), since this indicator is potentially significant for determining the prevalence of a number of hereditary diseases among the population. Studying the age at marriage allows us to identify unfavorable trends and risks of potential negative changes in the structure of gene pools (and accordingly, the level of health) of subsequent generations.

Genetika. 2024;60(10):90-102
pages 90-102 views

Hypermethylation of Long Non-Coding RNA Genes GAS5, Hotair, Hotairm1 and SSTR5-AS1 As Factors in the Development and Progression of Metastatic Breast Cancer

Filippova E., Lukina S., Loginov V., Burdennyy A., Pronina I., Arzhanukhina N., Kazubskaya T., Braga E.

Аннотация

Metastasis to the lymph nodes is one of the most important factors in the poor prognosis of patients with breast cancer; the five-year survival rate for metastatic breast cancer is less than 30%. DNA methylation occurs in the early stages of the development of cancer, and also plays an important role in the development of lymphatic metastases and can serve as a diagnostic and prognostic marker of malignant tumors, including breast cancer. The lncRNA genes GAS5, HOTAIR, HOTAIRM1, SSTR5-AS1, presumably hypermethylated in breast cancer and associated with epithelial-mesenchymal transition and metastasis, were bioinformatically selected. Quantitative methyl-specific PCR showed a statistically significant increase in the methylation level of these lncRNA genes in breast tumors compared to the paired norm. Hypermethylation of the HOTAIRM1 and SSTR5-AS1 genes in breast cancer was identified for the first time. Using statistical analysis, positive correlations were established between methylation levels for the GAS5-HOTAIR and SSTR5-AS1-HOTAIRM1 pairs. The result of co-methylation of GAS5 and HOTAIR in breast cancer is consistent with the bioinformatically predicted (using enrichment analysis and the ncPath database) participation of these lncRNAs in the regulation of common signaling pathways and biological processes. The level of methylation of the lncRNA genes HOTAIRM1 and SSTR5-AS1 is associated with indicators of breast cancer progression (stage of the tumor process, tumor size, presence of metastases in the lymph nodes). A model has been proposed for assessing the risk of developing lymphogenous metastases depending on the level of methylation of the HOTAIRM1 gene. Thus, data were obtained on lncRNAs GAS5, HOTAIR, HOTAIRM1 and SSTR5-AS1 and hypermethylation of their genes as factors in the development and progression of metastatic breast cancer.

Genetika. 2024;60(10):103-110
pages 103-110 views

КРАТКИЕ СООБЩЕНИЯ

Tetracycline Induction of Natural Drug Resistance to Bedaquiline in Mycobacterium smegmatis mc2 155

Vatlin A., Tsybizov D., Letvinova V., Danilenko V.

Аннотация

The emergence of antibiotic resistance in microorganisms, including mycobacteria, poses a serious problem in modern medicine, reducing treatment effectiveness. In the modern world, there is considerable discussion about the influence of minimal selective concentrations of antibiotics (MSC), which are significantly lower than classical minimal inhibitory concentrations (MIC), on the emergence of antibacterial resistance. It is assumed that such microconcentrations may act as an additional mechanism for selecting drug-resistant strains, which is particularly relevant due to the accumulation of antibiotic concentrations in the environment as a result of human activity. In the context of mycobacteria, understanding the processes of induction of resistance to antibiotics at the MSC level is especially important for the development of effective treatment strategies and control of the spread of drug resistance. The aim of this study was to investigate the induction of the natural drug resistance system in mycobacteria under the influence of concentrations significantly lower than standard MIC and not affecting cell growth. The resistance of Mycobacterium smegmatis mc2 155 to one of the main antibiotics used in medical practice, bedaquiline, was analyzed during induction by tetracycline, ofloxacin, and kanamycin. It was established that one of the mechanisms influencing the change in sensitivity of the M. smegmatis mc2 155 strain during induction by microconcentrations of tetracycline is the antibiotic efflux system – MmpS5-Mmpl5.

Genetika. 2024;60(10):111-116
pages 111-116 views

Transferability of SSR Markers Developed on Gosseberry to Red and Black Currants

Pikunova A., Pavlenko A., Dolzhikova M., Golyaeva O., Knyazev S.

Аннотация

Eleven microsatellite markers previously developed based on gooseberry sequences were tested on red and black currants. As a result, all microsatellite loci were amplified on representatives of black currant, but on representatives of red currant there was no amplification in three loci (RucANS, RucDFR2-1, RucDFR1-3). Polymorphic loci have been identified for both black and red currants. In the MTT-7 locus in the studied black currant genotypes, amplification of 3 fragments has been observed. Probably this locus is duplicated in black currant genome. At the same time, in red currant, amplification typical for monolocus microsatellite has been observed in the MTT-7 locus. The RucHLH-1 and RucUFGT loci were tested on a hybrid red currant family (Belaya Potapenko × 1426-21-80). By genetic mapping, the localization of the RucHLH-1 locus was established on linkage group 4 in the red currant genome, and the localization of RucUFGT was presumably on linkage group 1. The set of microsatellite loci for the currant genus is currently limited. This work shows that some of the SSR markers developed on gooseberries are amplified and reveal polymorphism in currants and can be used to study both black and red currants.

Genetika. 2024;60(10):117-121
pages 117-121 views

The shaggy Gene Encoding the GSK3 Protein Kinase Controls the Sex-Dependent Effects of Specific Clusters of D. melanogaster Dopaminergic Neurons on Lifespan

Roshina N., Veselkina E., Trostnikov M., Pasyukova E.

Аннотация

Dopaminergic neurons control behavior, memory, and locomotion, and the causal relationship of their dysfunction to neurodegenerative diseases and aging has drawn attention to investigating the involvement of dopaminergic neurons in the regulation of lifespan. The highly conserved serine-threonine protein kinase GSK3 (Glycogen Syntase Kinase 3), one of the most important multifunctional cellular enzymes in higher organisms, which in Drosophila melanogaster is encoded by the shaggy gene, plays an important role in the function of dopaminergic neurons. This paper provides the first evidence that altering shaggy expression levels in just a few clusters of dopaminergic neurons can affect lifespan. This effect can be either negative or positive and depends on sex. The data obtained may serve as a basis for further search for targeted cell-specific factors regulating the rate of aging, as well as for the development of highly specific approaches to the therapy of neurodegenerative diseases.

Genetika. 2024;60(10):122-128
pages 122-128 views

The genome of Staphylococcus epidermidis Isolated from Caseous Tuberculoma

Sinkov V., Orlova E., Ogarkov O., Suzdalnitsky A., Kondratov I., Belkova N., Rychkova L.

Аннотация

A number of facultative-anaerobic lipophilic microorganisms, including representatives of Corynebacterium and Staphylococcaceae, inhabit the necrotic contents of tuberculomas. A strain of Staphylococcus epidermidis was isolated from caseum, fully genome sequenced, and gene-mapped. The coagulase-negative Staphylococcus belonged to the MLST 73 genotype and was resistant to two antituberculosis drugs. The strain phenotypically had urease, gelatinase and beta-hemolytic activities and possessed the corresponding genes. Similar to S. epidermidis O47, its genome consists of a single chromosome containing approximately 2.4 million base pairs, and oriC has the same orientation. A total of 2333 genes were identified, of which 2206 were coding genes. In contigs of the genome, sequences of plasmid replication genes were found: rep7a, rep13, rep5b and pSK1. Phylogenetic analysis indicates the closeness of the analyzed genome with a large group of European strains. Considering the biochemical and microbiological properties of the isolated strain, we hypothesize that staphylococci and other facultative-anaerobic satellite microorganisms of tuberculosis foci may play an important role in caseum liquefaction due to their own proteolytic activity and attraction of neutrophils to the focus of inflammation.

Genetika. 2024;60(10):129-134
pages 129-134 views