Transferability of SSR Markers Developed on Gosseberry to Red and Black Currants
- Authors: Pikunova A.V.1, Pavlenko A.A.1, Dolzhikova M.A.1, Golyaeva O.D.1, Knyazev S.D.1
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Affiliations:
- All-Russian Research Institute of Fruit Crop Breeding, VNIISPK
- Issue: Vol 60, No 10 (2024)
- Pages: 117-121
- Section: КРАТКИЕ СООБЩЕНИЯ
- URL: https://rjpbr.com/0016-6758/article/view/667188
- DOI: https://doi.org/10.31857/S0016675824100114
- EDN: https://elibrary.ru/wekcgy
- ID: 667188
Cite item
Abstract
Eleven microsatellite markers previously developed based on gooseberry sequences were tested on red and black currants. As a result, all microsatellite loci were amplified on representatives of black currant, but on representatives of red currant there was no amplification in three loci (RucANS, RucDFR2-1, RucDFR1-3). Polymorphic loci have been identified for both black and red currants. In the MTT-7 locus in the studied black currant genotypes, amplification of 3 fragments has been observed. Probably this locus is duplicated in black currant genome. At the same time, in red currant, amplification typical for monolocus microsatellite has been observed in the MTT-7 locus. The RucHLH-1 and RucUFGT loci were tested on a hybrid red currant family (Belaya Potapenko × 1426-21-80). By genetic mapping, the localization of the RucHLH-1 locus was established on linkage group 4 in the red currant genome, and the localization of RucUFGT was presumably on linkage group 1. The set of microsatellite loci for the currant genus is currently limited. This work shows that some of the SSR markers developed on gooseberries are amplified and reveal polymorphism in currants and can be used to study both black and red currants.
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About the authors
A. V. Pikunova
All-Russian Research Institute of Fruit Crop Breeding, VNIISPK
Author for correspondence.
Email: pikuanna84@mail.ru
Russian Federation, Zhilina, Orel district, 302530
A. A. Pavlenko
All-Russian Research Institute of Fruit Crop Breeding, VNIISPK
Email: pikuanna84@mail.ru
Russian Federation, Zhilina, Orel district, 302530
M. A. Dolzhikova
All-Russian Research Institute of Fruit Crop Breeding, VNIISPK
Email: pikuanna84@mail.ru
Russian Federation, Zhilina, Orel district, 302530
O. D. Golyaeva
All-Russian Research Institute of Fruit Crop Breeding, VNIISPK
Email: pikuanna84@mail.ru
Russian Federation, Zhilina, Orel district, 302530
S. D. Knyazev
All-Russian Research Institute of Fruit Crop Breeding, VNIISPK
Email: pikuanna84@mail.ru
Russian Federation, Zhilina, Orel district, 302530
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