The genome of Staphylococcus epidermidis Isolated from Caseous Tuberculoma
- Authors: Sinkov V.V.1, Orlova E.A.1, Ogarkov O.B.1, Suzdalnitsky A.E.2,3, Kondratov I.G.1, Belkova N.L.1, Rychkova L.V.1
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Affiliations:
- Scientific Сentre for Family Health and Human Reproduction Problems
- Irkutsk Tuberculosum-prevention hospital
- Irkutsk State Medical University
- Issue: Vol 60, No 10 (2024)
- Pages: 129-134
- Section: КРАТКИЕ СООБЩЕНИЯ
- URL: https://rjpbr.com/0016-6758/article/view/667191
- DOI: https://doi.org/10.31857/S0016675824100139
- EDN: https://elibrary.ru/wefylg
- ID: 667191
Cite item
Abstract
A number of facultative-anaerobic lipophilic microorganisms, including representatives of Corynebacterium and Staphylococcaceae, inhabit the necrotic contents of tuberculomas. A strain of Staphylococcus epidermidis was isolated from caseum, fully genome sequenced, and gene-mapped. The coagulase-negative Staphylococcus belonged to the MLST 73 genotype and was resistant to two antituberculosis drugs. The strain phenotypically had urease, gelatinase and beta-hemolytic activities and possessed the corresponding genes. Similar to S. epidermidis O47, its genome consists of a single chromosome containing approximately 2.4 million base pairs, and oriC has the same orientation. A total of 2333 genes were identified, of which 2206 were coding genes. In contigs of the genome, sequences of plasmid replication genes were found: rep7a, rep13, rep5b and pSK1. Phylogenetic analysis indicates the closeness of the analyzed genome with a large group of European strains. Considering the biochemical and microbiological properties of the isolated strain, we hypothesize that staphylococci and other facultative-anaerobic satellite microorganisms of tuberculosis foci may play an important role in caseum liquefaction due to their own proteolytic activity and attraction of neutrophils to the focus of inflammation.
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About the authors
V. V. Sinkov
Scientific Сentre for Family Health and Human Reproduction Problems
Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664003
E. A. Orlova
Scientific Сentre for Family Health and Human Reproduction Problems
Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664003
O. B. Ogarkov
Scientific Сentre for Family Health and Human Reproduction Problems
Author for correspondence.
Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664003
A. E. Suzdalnitsky
Irkutsk Tuberculosum-prevention hospital; Irkutsk State Medical University
Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664039; Irkutsk, 664003
I. G. Kondratov
Scientific Сentre for Family Health and Human Reproduction Problems
Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664003
N. L. Belkova
Scientific Сentre for Family Health and Human Reproduction Problems
Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664003
L. V. Rychkova
Scientific Сentre for Family Health and Human Reproduction Problems
Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664003
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