Comparative Analysis of Mutations of the Gynogenesis Maize Genes

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Abstract

The SNP polymorphism in gynogenesis (Zm_Pla1, Zm_CenH3, Zm_Dmp7) genes from haploid-inducing and control maize lines was evaluated. Using sequencing and multiple alignment methods the SNP, deletions, insertions in the target genes was established in comparing with the reference B73 maize line, and the phylogenetic trees were prepared. The presence of a 4-nucleotide insertions (4NI) and 15 identical SNP in the Zm_Pla1 gene of the ancestral Stock 6 and haploid-inducing ZMS-8 and ZMS-P maize lines and the absence of 4NI in the Zm_Pla1 gene in parthenogenetic as well as control maize lines were established. Phylogenetic analysis of the Zm_Pla1 gene confirmed the relationships between Stock 6 and haploid-inducing ZMS-P and ZMS-8 maize lines from Saratov. The presence of 5 SNPs in the Zm_Dmp7 gene of the ZMS-8 line, 3 SNPs and one 3-nucleotide deletion in the Zm_Dmp7 gene of the ZMS-P line, 3 SNPs and a 9-nucleotide deletion in the Zm_Dmp7 gene of the KM line were observed. The SNP (at position 131 from the start codon) in the Zm_Dmp7 gene is the reason for the increased haploid-inducing ability in CAU5, but not for ZMS-P maize line. Additionally, the 3-nucleotide deletion in the Zm_Dmp7 gene in the ZMS-P maize line and a 9-nucleotide deletion in the KM maize line were observed.

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About the authors

Ye. M. Moiseeva

Institute of Biochemistry and Physiology of Plants and Microorganisms, Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”

Email: chumakov_m@ibppm.ru
Russian Federation, Saratov, 410049

V. V. Fadeev

Institute of Biochemistry and Physiology of Plants and Microorganisms, Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”

Email: chumakov_m@ibppm.ru
Russian Federation, Saratov, 410049

Yu. V. Fadeeva

Institute of Biochemistry and Physiology of Plants and Microorganisms, Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”

Email: chumakov_m@ibppm.ru
Russian Federation, Saratov, 410049

S. I. Mazilov

Institute of Biochemistry and Physiology of Plants and Microorganisms, Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”

Email: chumakov_m@ibppm.ru
Russian Federation, Saratov, 410049

M. I. Chumakov

Institute of Biochemistry and Physiology of Plants and Microorganisms, Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”

Author for correspondence.
Email: chumakov_m@ibppm.ru
Russian Federation, Saratov, 410049

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Supplementary files

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2. Fig. 1. Multiple alignment of nucleotide sequences of the Zm_Pla1 gene in the Saratov-bred maize lines KM, ZMS-8, ZMS-P, GPL-1, as well as the Stock 6 and B73 lines, performed using the BLAST program. Dots indicate nucleotide similarity in the maize lines, letters indicate single-nucleotide substitutions, and a hyphen indicates the absence of a nucleotide.

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3. Fig. 2. Multiple alignment of nucleotide sequences of the Zm_Dmp7 gene in the KM, ZMS-8, ZMS-P maize lines of Saratov selection and the B73 reference line, performed using the BLAST program. Dots indicate the nucleotide similarity of the Zm_Dmp7 gene sequences of the studied lines with the Zm_Dmp7 gene sequence of the B73 reference line; ONS in the KM, ZMS-8 and ZMS-P lines are designated by letters; a hyphen means the absence of a nucleotide. The ONS of the ZMS-8 line, which coincides with the ONS of the CAU5 line, is underlined.

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4. Fig. 3. Phylogram of maize lines for the Zm_Pla1 gene, constructed in the MEGA 11 program [by 16] (line designations are given according to the author's original). A version of the presentation with evolutionary distance data for each of the branches; the studied lines of Saratov selection are highlighted in frames.

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5. Fig. 4. Phylogram of maize lines for the Zm_DMP7 gene, constructed in the MEGA 11 program [by 16] (line designations are given according to the author's original). A version of the presentation with evolutionary distance data for each of the branches; the studied lines of Saratov selection are highlighted in frames.

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