Transferability of SSR Markers Developed on Gosseberry to Red and Black Currants

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Eleven microsatellite markers previously developed based on gooseberry sequences were tested on red and black currants. As a result, all microsatellite loci were amplified on representatives of black currant, but on representatives of red currant there was no amplification in three loci (RucANS, RucDFR2-1, RucDFR1-3). Polymorphic loci have been identified for both black and red currants. In the MTT-7 locus in the studied black currant genotypes, amplification of 3 fragments has been observed. Probably this locus is duplicated in black currant genome. At the same time, in red currant, amplification typical for monolocus microsatellite has been observed in the MTT-7 locus. The RucHLH-1 and RucUFGT loci were tested on a hybrid red currant family (Belaya Potapenko × 1426-21-80). By genetic mapping, the localization of the RucHLH-1 locus was established on linkage group 4 in the red currant genome, and the localization of RucUFGT was presumably on linkage group 1. The set of microsatellite loci for the currant genus is currently limited. This work shows that some of the SSR markers developed on gooseberries are amplified and reveal polymorphism in currants and can be used to study both black and red currants.

Толық мәтін

Рұқсат жабық

Авторлар туралы

A. Pikunova

All-Russian Research Institute of Fruit Crop Breeding, VNIISPK

Хат алмасуға жауапты Автор.
Email: pikuanna84@mail.ru
Ресей, Zhilina, Orel district, 302530

A. Pavlenko

All-Russian Research Institute of Fruit Crop Breeding, VNIISPK

Email: pikuanna84@mail.ru
Ресей, Zhilina, Orel district, 302530

M. Dolzhikova

All-Russian Research Institute of Fruit Crop Breeding, VNIISPK

Email: pikuanna84@mail.ru
Ресей, Zhilina, Orel district, 302530

O. Golyaeva

All-Russian Research Institute of Fruit Crop Breeding, VNIISPK

Email: pikuanna84@mail.ru
Ресей, Zhilina, Orel district, 302530

S. Knyazev

All-Russian Research Institute of Fruit Crop Breeding, VNIISPK

Email: pikuanna84@mail.ru
Ресей, Zhilina, Orel district, 302530

Әдебиет тізімі

  1. Vieira M.L., Santini L., Diniz A.L., Munhoz C.D. Microsatellite markers: What they mean and why they are so useful // Genet. Mol. Biol. 2016. V. 4. № 39. P. 312–328. https://doi.org/10.1590/1678-4685-GMB-2016-0027
  2. Brennan R., Jorgensen L., Woodhead M., Russell J. Development and characterization of SSR markers in Ribes species // Mol. Ecol. 2002. V. 2. № 3. P. 327–330. https://doi.org/10.1046/j.1471-8286.2002.00233.х
  3. Brennan R., Jorgensen L., Hackett C. et al. The development of a genetic linkage map of blackcurrant (Ribes nigrum L.) and the identification of regions associated with key fruit quality and agronomic traits // Euphytica. 2008. V. 161. P. 19–34. https://doi.org/10.1007/s10681-007-9412-8
  4. Пикунова А.В, Горюнова С.В, Горюнов Д.В. Генетическая карта смородины красной (Ribes rubrum L.), построенная с применением SSR и SNP ДНК-маркеров // Генетика. 2020. V. 56. № 11. С. 1340–1344. https://doi.org/10.31857/S0016675820100100
  5. Kalia R.K., Rai M.K., Kalia S. et al. Microsatellite markers: An overview of the recent progress in plants // Euphytica. 2011. V. 177. № 3. P. 309–334. https://doi.org/10.1007/s10681-010-0286-9
  6. Князев С.Д., Огольцова Т.П. Селекция смородины черной на современном этапе. Орел: Изд-во ОрелГАУ, 2004. 238 с.
  7. Janczewski E. Monograph of the currants Ribes L. // Mem. Soc. Phys. Hist. Nat. Geneve. 1907. V. 35. P. 199–517.
  8. Berger A. A taxonomic review of currants and gooseberries // N.Y. Agric. Exptl Sta. Techn. Bull. 1924. V. 109. P. 1–118.
  9. Doyle J.J., Doyle J.L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue // Phytochem. Bull. 1987. V. 19. P. 11–15.
  10. Antonius K., Karhu S., Kaldm H. et al. Development of the Northern European Ribes core collection based on a microsatellite (SSR) marker diversity analysis // Plant Genet. Res.: Characterization and Utilization. 2012. V. 10. P. 70–73. https://doi.org/10.1017/S1479262111000980
  11. Vidyagina E.O., Lebedev V.G., Subbotina N.M. The development of the genic SSR markers for analysis of genetic diversity in gooseberry cultivars // Agronomy. 2021. V. 11. № 6. https://doi.org/10.3390/agronomy11061050
  12. Peakall R., Smouse P.E. GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research — an update // Bioinformatics. 2012. V. 28. Р. 2537–2539. https: 10.1111/j.1471- 8286.2005.01155.x
  13. Пикунова А.В., Мартиросян Е.В., Князев С.Д., Рыжова Н.Н. Применение RAPD-анализа для изучения генетического полиморфизма и филогенетических связей у представителей рода Ribes L // Экол. генетика. 2011. Т. 9. № 2. С. 34–44.
  14. Pikunova A., Goryunova S., Goryunov D. Genetic diversity and pedigree analysis of red currant germplasm // Plants. 2022. V. 11. № 13. https://doi.org/10.3390/plants11131623

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2. Fig. 1. Genetic map of linkage group 4 of red currant and localization of the RucHLH-1 locus on it (indicated by arrow).

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