Polymorphism of microsatellite loci in populations of Caucasian rock lizards and its use for assessing the genetic diversity of Darevskia raddei

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Abstract

The study of Caucasian rock lizards of the Darevskia raddei complex, represented by several subspecies, is of great interest and scientific significance in connection with their participation in interspecific hybridizations with the formation of five of the seven known unisexual (parthenogenetic) species of the genus Darevskia. Here are presented genetic parameters for populations (subspecies) of D. r. raddei and D. r. nairensis based on the analysis of the variability of 10 microsatellite loci of 230 individuals from 17 populations of Armenia and Nagorno-Karabakh. According to these parameters, D. r. raddei are characterized by greater diversity in the number of alleles and genotypes compared to D. r. nairensis. Genetic differentiation analysis showed that D. r. raddei populations are divided into two groups, one of which is genetically closer to D. r. nairensis than D. r. raddei. Analysis of the association index showed the absence of free recombination of alleles between subspecies, which indicates their isolation and the absence of crossing between individuals. Thus, based on the expanded population sample and the developed panel of microsatellite markers, new data on the population structure of D. raddei species, genetic diversity and differentiation of D. r. raddei and D. r. nairensis were obtained.

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About the authors

D. O. Odegov

Institute of Gene Biology Russian Academy of Sciences

Author for correspondence.
Email: irena-m@yandex.ru
Russian Federation, Moscow, 117334

A. A. Valyaeva

Institute of Gene Biology Russian Academy of Sciences

Email: irena-m@yandex.ru
Russian Federation, Moscow, 117334

M. S. Arakelyan

Yerevan State University

Email: irena-m@yandex.ru
Armenia, Erevan, 0025

A. P. Ryskov

Institute of Gene Biology Russian Academy of Sciences

Email: irena-m@yandex.ru
Russian Federation, Moscow, 117334

V. I. Korchagin

Institute of Gene Biology Russian Academy of Sciences

Email: irena-m@yandex.ru
Russian Federation, Moscow, 117334

I. A. Martirosyan

Institute of Gene Biology Russian Academy of Sciences

Email: irena-m@yandex.ru
Russian Federation, Moscow, 117334

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Supplementary files

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1. JATS XML
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2. Fig. 1. The number of homozygous individuals at the microsatellite locus sc12962 in the samples of D. raddei and D. valentini.

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3. Fig. 2. The frequency of null alleles at each locus in the D. raddei and D. valentine samples.

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4. Fig. 3. An unrooted phylogenetic tree of D. raddei populations, built on the basis of genetic distances along It with bootstrap support in 100 iterations.

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5. Fig. 4. Index of the association ( ) of subspecies of D. raddei. a – without division into subspecies; b – D. R. raddei; c – D. R. nairensis.

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6. Fig. 5. Index of association () of population groups D. R. raddei GG (a) and D. R. raddei TT (b).

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7. Fig. 6. Clustering of individuals of D. raddei and D. valentini species based on discriminant analysis of the main components. a – the number of clusters K = 3; b – the number of clusters K = 4.

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8. Table 1. Localities of biological material collection. N is the number of samples
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