Meta-analysis of the Mesenchymal Stem Cells Immortalization Protocols: A Guideline for Regenerative Medicine
- Авторлар: Elias Ferreira Stricker P.1, de Oliveira N.1, Felipe Mogharbel B.1, Lührs L.1, Irioda A.C.1, Abdelwahid E.1, Regina Cavalli L.1, Zotarelli-Filho I.1, de Carvalho K.A.1
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Мекемелер:
- , Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties
- Шығарылым: Том 19, № 7 (2024)
- Беттер: 1009-1020
- Бөлім: Medicine
- URL: https://rjpbr.com/1574-888X/article/view/645887
- DOI: https://doi.org/10.2174/011574888X268464231016070900
- ID: 645887
Дәйексөз келтіру
Толық мәтін
Аннотация
Background::This systematic review describes the most common methodologies for immortalizing human and animal mesenchymal stem cells (MSCs). This study follows the rules of PRISMA and is registered in the Institutional Review Board of PROSPERO International of systematic reviews, numbered protocol code: CRD42020202465.
Method::The data search systematization was based on the words "mesenchymal stem cell" AND "immortalization." The search period for publications was between 2000 and 2022, and the databases used were SCOPUS, PUBMED, and SCIENCE DIRECT. The search strategies identified 384 articles: 229 in the SCOPUS database, 84 in PUBMED, and 71 in SCIENCE DIRECT. After screening by titles and abstracts, 285 articles remained. This review included thirty-nine articles according to the inclusion and exclusion criteria.
Result::In 28 articles, MSCs were immortalized from humans and 11 animals. The most used immortalization methodology was viral transfection. The most common immortalized cell type was the MSC from bone marrow, and the most used gene for immortalizing human and animal MSCs was hTERT (39.3%) and SV40T (54.5%), respectively.
Conclusion::Also, it was observed that although less than half of the studies performed tumorigenicity assays to validate the immortalized MSCs, other assays, such as qRT-PCR, colony formation in soft agar, karyotype, FISH, and cell proliferation, were performed in most studies on distinct MSC cell passages.
Негізгі сөздер
Авторлар туралы
Priscila Elias Ferreira Stricker
, Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties
Email: info@benthamscience.net
Nathalia de Oliveira
, Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties
Email: info@benthamscience.net
Bassam Felipe Mogharbel
, Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties
Email: info@benthamscience.net
Larissa Lührs
, Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties
Email: info@benthamscience.net
Ana Irioda
, Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties
Email: info@benthamscience.net
Eltyeb Abdelwahid
, Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties
Email: info@benthamscience.net
Luciane Regina Cavalli
, Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties
Email: info@benthamscience.net
Idiberto Zotarelli-Filho
, Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties
Хат алмасуға жауапты Автор.
Email: info@benthamscience.net
Katherine de Carvalho
, Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties
Хат алмасуға жауапты Автор.
Email: info@benthamscience.net
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