TNF-α Pretreated Hematopoietic Stem Cells Inhibit the Migration and Inflammatory Response of HUVECs and Attenuate GVHD


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Abstract

Background:Hematologic diseases have seriously threatened human health. Although hematopoietic stem cell transplantation (HSCT) is an effective curative option, the complications, especially graft-versus-host disease (GVHD), are a big problem

Methods:TNF-α pretreatment of hematopoietic stem cells. Apoptosis was detected by flow cytometry, Transwell, and wound healing assays were used to assess cell migration and invasion, E-selectin expression was observed by fluorescence imaging, the levels of NO were measured by a kit, the expression of Ecadherin, MMP2, and MMP9 was detected in cells by qRT-PCR, and western blot was used to analyze the expression of E-cadherin, CXCL12, MCP-1, MCP-3, MMP2, and MMP9.

Results:TNF-α induces a high apoptosis rate of CD3, CD19, and CD133 and a low apoptosis rate of CD34. The level of Fas and TNF-R1 was significantly high than that of TNF-R2. HSCs treated with TNF- α declined the invasion and migration of HUVECs. E-selectin, MMP2 and MMP9 mRNA levels of HUVECs and MMP2, CXCL12, MCP-1, and MCP-3 were decreased after HSCs-TNF-α treatment, while the E-cadherin mRNA and protein level of HUVECs was enhanced with HSCs-TNF-α treatment.

Conclusion:TNF-α pretreated HSCs can lead to reduced levels of migration, adhesion, and chemokines of HUVECs, thereby declining the inflammatory response and GVHD.

About the authors

Jilei Sun

Department of Urology, The Affiliated Hospital of Changchun University of Chinese Medicine

Email: info@benthamscience.net

Tingting Zhou

Department of Urology, Western Theater General Hospital

Email: info@benthamscience.net

Shiyuan Qin

Department of Urology, Affiliated Hospital of Chengdu University

Email: info@benthamscience.net

Yaolei Zhang

Department of Urology, Western Theater General Hospital

Email: info@benthamscience.net

Yong Yang

Department of Urology, The Affiliated Hospital of Changchun University of Chinese Medicine

Email: info@benthamscience.net

Zhitao Wei

Department of Urology, The Affiliated Hospital of Changchun University of Chinese Medicine

Author for correspondence.
Email: info@benthamscience.net

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