Use of Mesenchymal Stem Cells in Experimental Ovarian Damage


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Abstract

Background:Bisphenol-A (BPA) has a well-proven deleterious effect on the hypothalamicpituitary- gonadal axis.

Objectives:The current study investigated the therapeutic potentials of mesenchymal stem cells (MSCs) in a murine model of BPA-induced ovarian damage.

Methods:Fifty adult female rats were divided into: Group 1; control group, Group IIa, IIb: rats were given oral gavage of BPA (25 and 50 mg/Kg body weight respectively) on a daily basis for 15 days, and Group IIIa, IIIb; rats were intravenously treated with of MSCs (106 cells) after receiving the last dose of BPA as in group II. Plasma and ovarian tissue levels of Malondialdehyde (MDA) and gonadal axis hormones were assessed. Apoptosis was evaluated by TUNNEL assay and by apoptosis markers (FAS, FASL, Caspase 3, SLTM). A histological examination of ovarian tissue was also conducted.

Results:BPA resulted in a significant elevation in plasma levels of LH, FSH, and ovarian tissue levels of MDA and a significant decrease in estradiol and progesterone. All genetic and protein markers of apoptosis were elevated in BPA treated group with decreased oestrogen receptor expression in the ovarian tissue. Increased apoptotic cells were confirmed by TUNEL assay. A high dose of BPA was able to increase the number of atretic follicles in the ovarian tissue whereas the numbers of primordial, primary, secondary and Graafian follicles were decreased. All the laboratory and histological abnormalities were ameliorated by treatment with MSCs.

Conclusion:The antioxidant and anti-apoptotic effects of MSCs could possibly explain the ability of this therapeutic modality to ameliorate BPA-induced-ovarian damage.

About the authors

Hanan Fouad

Department of Medical Biochemistry, Faculty of Medicine, Cairo University

Email: info@benthamscience.net

Ibrahim Albahlol

Department of Obstetrics and Gynecology, College of Medicine, Jouf University

Email: info@benthamscience.net

Hazim Wahab

Department of Histology, Faculty of Medicine, Menofiya University

Email: info@benthamscience.net

Eman Nadwa

Department of Pharmacology and Therapeutics, College of Medicine, Jouf University

Email: info@benthamscience.net

Heba Galal

Department of Medical Physiology, College of Medicine, Jouf University

Email: info@benthamscience.net

Mohamed Abouelkheir

Department of Pharmacology and Therapeutics, College of Medicine, Jouf University

Author for correspondence.
Email: info@benthamscience.net

Ahmed Taha

Microbiology and immunology unit, Department of Pathology, College of Medicine, Jouf University

Email: info@benthamscience.net

Abdelkarim Kamel

Basic Medical Sciences, Faculty of Medicine, Cairo University

Email: info@benthamscience.net

Hassan Abdelmawlla

Department of Anatomy, College of Medicine, Jouf University

Email: info@benthamscience.net

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