Differential Expression of microRNAs and Target Genes Analysis in Olfactory Ensheathing Cell-derived Extracellular Vesicles Versus Olfactory Ensheathing Cells


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Abstract

Introduction:Olfactory ensheathing cells (OECs) are important transplantable cells for the treatment of spinal cord injury. However, information on the mechanism of OEC-derived extracellular vesicles (EVs) in nerve repair is scarce.

Methods:We cultured OECs and extracted the OEC-derived EVs, which were identified using a transmission electron microscope, nanoparticle flow cytometry, and western blotting. High throughput RNA sequencing of OECs and OEC-EVs was performed, and the differentially expressed microRNAs (miRNAs) (DERs) were analyzed by bioinformatics. The target genes of DERs were identified using miRWalk, miRDB, miRTarBase, and TargetScan databases. Gene ontology and KEGG mapper tools were used to analyze the predicted target genes. Subsequently, the STRING database and Cytoscape software platform were used to analyze and construct miRNA target genes' protein-protein interaction (PPI) network.

Results:Overall, 206 miRNAs (105 upregulated and 101 downregulated) were differentially expressed in OEC-EVs (p < 0.05;⋅log2 (fold change)⋅>2). Six DERs (rno-miR-7a-5p, rno-miR-143-3p, rno-miR-182, rno-miR-214-3p, rno-miR-434-5p, rno-miR-543-3p) were significantly up-regulated , and a total of 974 miRNAs target genes were obtained. The target genes were mainly involved in biological processes such as regulation of cell size, positive regulation of cellular catabolic process and small GTPase-mediated signal transduction; positive regulation of genes involved in cellular components such as growth cone, site of polarized growth, and distal axon; and molecular functions such as small GTPase binding and Ras GTPase binding. In pathway analysis, target genes regulated by six DERs were mainly enriched in axon guidance, endocytosis, and Ras and cGMP-dependent protein kinase G signaling pathways. Finally, 19 hub genes were identified via the PPI network.

Conclusion:Our study provides a theoretical basis for treating nerve repair by OEC-derived EVs.

About the authors

Yubing Yang

Department of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University

Email: info@benthamscience.net

Jiaxi Li

Department of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University

Email: info@benthamscience.net

Weidong Liu

Department of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University,

Email: info@benthamscience.net

Dong Guo

Department of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University

Email: info@benthamscience.net

Zhengchao Gao

Department of Orthopedics, Shaanxi Provincial People's Hospital

Email: info@benthamscience.net

Yingjie Zhao

Department of Orthopedics, Second Affiliated Hospital of Xi'an Jiaotong University

Email: info@benthamscience.net

Minchao Zhao

Department of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University

Email: info@benthamscience.net

Xijing He

Department of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong Universit

Author for correspondence.
Email: info@benthamscience.net

Su’e Chang

Department of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University

Author for correspondence.
Email: info@benthamscience.net

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