Аnalyzing buckwheat genotypes obtained on selective media with zinc in vitro using ISSR markers

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Abstract

The paper evaluates four ISSR-markers (М1, М2, М7, and М11) for their effectiveness in research on the genetic distances of regenerated Fagopyrum esculentum plants, which were obtained in vitro on selective media with high doses of ZnSO4×7 H2O (808–1313 mg/L). The level of polymorphism was determined to be high in the combined sample (74.4%). The obtained speciemens might be used in the breeding of new varieties with economically important traits.

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About the authors

S. A. Borovaya

Chaikа Federal Scientific Center of Agricultural Biotechnology of the Far East

Author for correspondence.
Email: borovayasveta@mail.ru
Russian Federation, Primorsky Krai, Timiriasevsky, 692539

A. G. Klykov

Chaikа Federal Scientific Center of Agricultural Biotechnology of the Far East

Email: borovayasveta@mail.ru
Russian Federation, Primorsky Krai, Timiriasevsky, 692539

N. G. Boginskaya

Chaikа Federal Scientific Center of Agricultural Biotechnology of the Far East

Email: borovayasveta@mail.ru
Russian Federation, Primorsky Krai, Timiriasevsky, 692539

References

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Supplementary files

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2. Fig. 1. UPGMA is a dendrogram based on the values of genetic distances for buckwheat regenerants. 1 is a group of variants characterized by the smallest DN compared to the original form; 2 is a group of variants characterized by the largest DN compared to the original form.

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