Identification of ultrastructural details of the astrocyte process system in neural tissue of the brain using correlative scanning probe and transmission electron microscopy

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Nanoscale morphological features of branched processes of glial cells may be of decisive importance for neuron-astrocytic interactions in health and disease. The paper presents the results of a correlation analysis of images of thin processes of astrocytes in the nervous tissue of the mouse brain, obtained by scanning probe microscopy and transmission electron microscopy with high spatial resolution. Samples were prepared and imaged using a unique hardware combination of ultramicrotomy and scanning probe microscopy. It was shown that the images identified details of astrocytes with a thickness of the order of tens of nanometers, which can be used in the future to reconstruct the three-dimensional structure of astrocytic processes by integrating a series of sequential images of ultrathin sections of nervous tissue.

Толық мәтін

Рұқсат жабық

Авторлар туралы

О. Agapova

Shumakov National Medical Research Center of Transplantology and Artificial Organs

Email: igor.agapov@gmail.com
Ресей, Moscow

А. Efimov

Shumakov National Medical Research Center of Transplantology and Artificial Organs

Email: igor.agapov@gmail.com
Ресей, Moscow

E. Obraztsova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Moscow Institute of Physics and Technology

Email: igor.agapov@gmail.com
Ресей, Moscow; Dolgoprudny

K. Mochalov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: igor.agapov@gmail.com
Ресей, Moscow

D. Solovyeva

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: igor.agapov@gmail.com
Ресей, Moscow

V. Oleinikov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)

Email: igor.agapov@gmail.com
Ресей, Moscow; Moscow

I. Agapov

Shumakov National Medical Research Center of Transplantology and Artificial Organs

Хат алмасуға жауапты Автор.
Email: igor.agapov@gmail.com
Ресей, Moscow

S. Gautier

Shumakov National Medical Research Center of Transplantology and Artificial Organs; Sechenov University

Email: igor.agapov@gmail.com

Academician

Ресей, Moscow; Moscow

Әдебиет тізімі

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  8. Mochalov K.E., Chistyakov A.A., Solovyeva D.O., et al. An instrumental approach to combining confocal microspectroscopy and 3D scanning probe nanotomography // Ultramicroscopy. 2017. V. 182. P. 118–123.
  9. Миронов А.А., Комиссарчик Я.Ю., Миронов В.А. Методы электронной микроскопии в биологии и медицине / А.А. Миронов, Я.Ю. Комиссарчик, В.А. Миронов; отв. ред. Н.Н. Никольский. – СПб.: Наука, 1994.
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Жүктеу
2. Complementary TEM and SPM images of astrocyte areas. Overview images (left) and enlarged areas (right). In the magnified images, the thin processes of astrocytes in TEM images are marked with color, and symbols indicate complementary regions.

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