Reproductive technologies and Parkinson’s disease: experimental study of substantia nigra in the brain and motor functions on C57BL/6 and B6.CG-TG mice
- Authors: Kozeneva V.S.1,2, Rozhkova I.N.1, Brusentsev E.Y.1, Rakhmanova T.A.1,2, Shavshaeva N.A.1,2, Afanasova S.G.1,2, Lebedeva D.A.1, Okotrub S.V.1, Igonina T.N.1, Amstislavskya S.Y.1
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Affiliations:
- Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences
- Novosibirsk State University
- Issue: Vol 111, No 1 (2025)
- Pages: 33-53
- Section: EXPERIMENTAL ARTICLES
- URL: https://rjpbr.com/0869-8139/article/view/682949
- DOI: https://doi.org/10.31857/S0869813925010027
- EDN: https://elibrary.ru/ULBFBM
- ID: 682949
Cite item
Abstract
Parkinson’s disease (PD) is an age-related neurodegenerative pathology characterized by abnormalities of the brain's dopaminergic system, alpha-synucleinopathy and motor dysfunction. Possible association of assisted reproductive technologies (ARTs) with neuropathologies is discussed in medicine literature, but there is a lack of experimental studies addressing this issue. The current study investigates the effects of ARTs, i.e. in vitro culture of preimplantation embryos and embryo transfer (ET) on the features characteristic for PD in offspring: motor dysfunction, decrease of neuronal density, e.g. density of dopaminergic neurons, as well as alpha-synuclein accumulation in substantia nigra pars compacta (SNpc). Male offspring of the B6.Cg-Tg strain and C57BL/6 strain (hereinafter referred as wild type, WT) obtained by ART (groups B6.Cg-Tg ET and WT ET) or by natural mating (groups B6.Cg-Tg CTL and WT CTL) were tested at the age of six months. Motor coordination and body balance were studied using the rotarod test; the density of neurons, as well as the accumulation of alpha-synuclein in the SNpc were assessed by immunohistochemical method. It was shown that B6.Cg-Tg mice obtained without ART (B6.Cg-Tg CTL) are characterized by the low density of neurons, including dopaminergic ones, as well as the accumulation of alpha-synuclein in SNpc as compared to wild type mice (WT CTL). Wild-type offspring obtained by ART (WT ET group) were characterized by the impairment in motor coordination and body balance, as well as by the decrease in the density of neurons in the SNpc, including dopaminergic ones. Offspring of the B6.Cg-Tg strain obtained by ART (B6.Cg-Tg ET group) were characterized by an increased accumulation of alpha-synuclein in the SNpc. The results of our study indicate possible association between using of modern reproductive technologies and predisposition to the neurodegenerative process and manifestations of the features characteristic to PD phenotype in offspring.
Full Text

About the authors
V. S. Kozeneva
Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University
Email: amstis@yandex.ru
Russian Federation, Novosibirsk; Novosibirsk
I. N. Rozhkova
Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences
Email: amstis@yandex.ru
Russian Federation, Novosibirsk
E. Y. Brusentsev
Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences
Email: amstis@yandex.ru
Russian Federation, Novosibirsk
T. A. Rakhmanova
Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University
Email: amstis@yandex.ru
Russian Federation, Novosibirsk; Novosibirsk
N. A. Shavshaeva
Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University
Email: amstis@yandex.ru
Russian Federation, Novosibirsk; Novosibirsk
S. G. Afanasova
Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University
Email: amstis@yandex.ru
Russian Federation, Novosibirsk; Novosibirsk
D. A. Lebedeva
Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences
Email: amstis@yandex.ru
Russian Federation, Novosibirsk
S. V. Okotrub
Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences
Email: amstis@yandex.ru
Russian Federation, Novosibirsk
T. N. Igonina
Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences
Email: amstis@yandex.ru
Russian Federation, Novosibirsk
S. Y. Amstislavskya
Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences
Author for correspondence.
Email: amstis@yandex.ru
Russian Federation, Novosibirsk
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