STUDY OF THE LIPID PIGMENT COMPOSITION IN THE WHITE SEA ALGAE SACCHARINA LATISSIMA USING TLC AND MALDI-MS

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Abstract

The qualitative composition of the most important biologically active lipid substances-carotenoids and chlorophyll derivatives of the White sea algaeSaccharina latissimahas been studied. The lipid extract was separated by thin-layer chromatography (TLC) and examined by matrix-associated laser desorption-ionization with mass-spectrometric analysis (MALDI-MS). The extract contained fucoxanthin, fucoxanthinol, pheophytin a, pheophorbide a, as well as other carotenoids and chlorophylls, including those not described in the literature. The results obtained, which significantly expand the information about the composition of S. latissima pigments, can be used to standardize raw materials and preparations based on these algae.

About the authors

К. А. Krasnov

Institute of Toxicology of the Federal Medical Biological Agency

Author for correspondence.
Email: krasnov_tox@mail.ru

Krasnov Konstantin Andreevich

192019, Saint Petersburg

Russian Federation

A. S. Gladchuk

Institute of Toxicology of the Federal Medical Biological Agency; St. Petersburg State University

Email: aleglad24@gmail.com

Gladchuk Aleksey Sergeevich

192019, Saint Petersburg

199034, Saint Petersburg

Russian Federation

M. L. Alexandrova

Institute of Toxicology of the Federal Medical Biological Agency

Email: analekt@mail.ru

Alexandrova Marina Leonidovna

192019, Saint Petersburg

Russian Federation

O. A. Keltsieva

Institute of Analytical Instrumentation, Russian Academy of Sciences; Institute of Toxicology of the Federal Medical Biological Agency

Email: keltcieva@gmail.com

Keltsieva Olga Alexandrovna

190103, Saint Petersburg

192019, Saint Petersburg

Russian Federation

M. A. Zaytseva

Institute of Toxicology of the Federal Medical Biological Agency

Email: alpha-2@mail.ru

Zaytseva Maria Anatolevna

192019, Saint Petersburg

Russian Federation

M. V. Melnikova

Institute of Toxicology of the Federal Medical Biological Agency

Email: margarita10108@mail.ru

Melnikova Margarita Viktorovna

192019, Saint Petersburg

Russian Federation

V. L. Reinyuk

Institute of Toxicology of the Federal Medical Biological Agency

Email: vladton@mail.ru

Reynyuk Vladimir Leonidovich

192019, Saint Petersburg

Russian Federation

Е. P. Podolskaya

Institute of Toxicology of the Federal Medical Biological Agency; Institute of Analytical Instrumentation, Russian Academy of Sciences

Email: ek.podolskaya@gmail.com

Podolskaya Ekaterina Petrovna

192019, Saint Petersburg

190103, Saint Petersburg

Russian Federation

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Copyright (c) 2020 Krasnov К.А., Gladchuk A.S., Alexandrova M.L., Keltsieva O.A., Zaytseva M.A., Melnikova M.V., Reinyuk V.L., Podolskaya Е.P.



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