CYTOME ANALYSIS OF EFFECTS OF GENOMIC INSTABILITY INDUCED BY DIFFERENTLY SIZED SILVER NANOPARTICLES IN HUMAN WHOLE BLOOD CULTURE

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Abstract

A high bactericidal activity of silver nanoparticles (NHS) suggests the possibility of their use in the preparation of drinking water. On the example of human peripheral blood lymphocytes cultured in micronucleus test with cytochalasin B, effects of genome instability were investigated in a concentration range of 0.005-5.0 mg / l of silver nanoparticles (NHS) with a size of 14.3 ± 0.2 nm (Ag14) and 100.0 ± 11.0 nm (AG100), stabilized with gum acacia and for comparison , those of Ag2SO4 (silver ions). In the same range of concentrations . All substances were suspended or dissolved in drinking water.The results showed that the NPS had genotoxic and cytotoxic effects that made them unsuitable to improve the quality of drinking water. Effects of genome instability determined by the frequency of dividing cells with micronuclei and nucleoplasmic bridges, as well as inhibition of mitotic activity, decrease of proliferative activity and increasing duration of the cell cycle came down in series of Ag2SO4 >> Ag100 >> Ag14. However, frequency rise of asymmetric 3-nucleated cells due to aneuploidy induction was the most characteristic of Ag14 particles. 

About the authors

F. I. Ingel

A.N. Sysin Research Institute of Human Ecology and Environmental Health, of Ministry of Health of Russia

Author for correspondence.
Email: fainaingel@mail.ru
119121, Moscow Russian Federation

E. K. Krivtsova

A.N. Sysin Research Institute of Human Ecology and Environmental Health, of Ministry of Health of Russia

Email: e_k_krivcova@mail.ru
119121, Moscow Russian Federation

N. A. Yurtseva

A.N. Sysin Research Institute of Human Ecology and Environmental Health, of Ministry of Health of Russia

Email: urtseva@mail.ru
119121, Moscow Russian Federation

O. N. Savostikiva

A.N. Sysin Research Institute of Human Ecology and Environmental Health, of Ministry of Health of Russia

Email: savon05@yandex.ru
119121, Moscow Russian Federation

A. V. Alekseeva

A.N. Sysin Research Institute of Human Ecology and Environmental Health, of Ministry of Health of Russia

Email: al.anna@list.ru
119121, Moscow Russian Federation

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Copyright (c) 2017 Ingel F.I., Krivtsova E.K., Yurtseva N.A., Savostikiva O.N., Alekseeva A.V.


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