电邮这篇文章 Rapid detection of A-type botulinum toxin using an aptasensor and SERS
- 作者: Ambartsumyan O.A.1, Brovko A.M.1
-
隶属关系:
- Moscow Institute of Physics and Technology
- 期: 卷 88, 编号 2 (2024)
- 页面: 219-226
- 栏目: New Materials and Technologies for Security Systems
- URL: https://rjpbr.com/0367-6765/article/view/654753
- DOI: https://doi.org/10.31857/S0367676524020097
- EDN: https://elibrary.ru/RSHEGU
- ID: 654753
如何引用文章
开放存取
##reader.subscriptionAccessGranted##
详细
We described the development of a biosensor for the rapid and sensitive detection of botulinum toxin type A. The sensor is a SERS substrate with an optimized concentration of labeled aptamers, immobilized on its surface. It allows the detection of botulinum toxin type A with a detection limit of 2.4 ng/ml in 1 hour.
关键词
全文:
作者简介
O. Ambartsumyan
Moscow Institute of Physics and Technology
编辑信件的主要联系方式.
Email: ambartsumian.oa@mipt.ru
俄罗斯联邦, Moscow
A. Brovko
Moscow Institute of Physics and Technology
Email: ambartsumian.oa@mipt.ru
俄罗斯联邦, Moscow
参考
- Pirazzini M., Rossetto O., Eleopra R., Montecuccco C. // Pharmacol. Rev. 2017. V. 69. P. 200.
- Smith T.J., Hill K.K., Raphael B.H. // Res. Microbiol. 2015. V. 166. P. 290.
- Rummel A. // Toxicon. 2015. V. 107. P. 9.
- Tian D., Zheng T. // PLoS One. 2014. V. 9. P. 1.
- Hill K.K., Smith T.J., Helma C.H. et al. // J. Bacteriol. 2007. V. 189. No. 3. P. 818.
- Strotmeier J., Willjes G., Binz T., Rummel A. // FEBS Lett. 2012. V. 586. P. 310.
- Kavalali E.T. // Nature. Rev. Neurosci. 2015. V. 16. P. 5.
- Kamińska A., Winkler K., Kowalska A. et al. // Sci. Rеports. 2017. V. 7. Art. No. 10656.
- Lim C.Y., Granger J.H., Porter M. // Analyt. Chim. Acta X. 2019. V. 1. Art. No. 100002.
- Kim K., Choi N., Jeon J.H. et al. // Sensors. 2019. V. 19. Art. No. 4081.
- Yoo H., Jo H., Oh S.S. // Mater. Adv. 2020. V. 1. P. 2663.
- Frevert J. // Drugs R D. 2010. V. 10. P. 67.
补充文件
附件文件
动作
1.
JATS XML
2.
Fig. 1. Schematic diagram of the experiment
下载 (153KB)
3.
Fig. 2. Ratio of SERS signal intensity of experiment and control
下载 (111KB)
4.
Fig. 3. Distribution of SERS signal intensity from the aptamer solution to BoNT with concentration of 35.0 nM on the substrate
下载 (167KB)
5.
Fig. 4. SERS signal intensity distribution from the aptamer solution to BoNT with 17.5 nM concentration on the substrate
下载 (121KB)
6.
Fig. 5. SERS signal intensity distribution from the aptamer solution to BoNT with 8.8 nM concentration on the substrate
下载 (134KB)
7.
Fig. 6. SERS signal intensity distribution of experiment and control for BoNT A (2.4 ng/ml) detection using a solution of aptamers with a concentration of 17.5 nM on the substrate
下载 (197KB)
8.
Fig. 7. Distribution of SERS intensity of experiment and control in the determination of IgGHum (24 ng/ml) using a solution of aptamers to BoNT with a concentration of 17.5 nM on the substrate
下载 (170KB)
9.
Fig. 8. Distribution of SERS intensity of the experiment and control in the determination of BoNT A (2.4 ng/ml) using a solution of aptamers to RSV with a concentration of 17.5 nM
下载 (189KB)
