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Rapid detection of A-type botulinum toxin using an aptasensor and SERS
- Authors: Ambartsumyan O.A.1, Brovko A.M.1
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Affiliations:
- Moscow Institute of Physics and Technology
- Issue: Vol 88, No 2 (2024)
- Pages: 219-226
- Section: New Materials and Technologies for Security Systems
- URL: https://rjpbr.com/0367-6765/article/view/654753
- DOI: https://doi.org/10.31857/S0367676524020097
- EDN: https://elibrary.ru/RSHEGU
- ID: 654753
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Abstract
We described the development of a biosensor for the rapid and sensitive detection of botulinum toxin type A. The sensor is a SERS substrate with an optimized concentration of labeled aptamers, immobilized on its surface. It allows the detection of botulinum toxin type A with a detection limit of 2.4 ng/ml in 1 hour.
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About the authors
O. A. Ambartsumyan
Moscow Institute of Physics and Technology
Author for correspondence.
Email: ambartsumian.oa@mipt.ru
Russian Federation, Moscow
A. M. Brovko
Moscow Institute of Physics and Technology
Email: ambartsumian.oa@mipt.ru
Russian Federation, Moscow
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Supplementary files
Supplementary Files
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Fig. 1. Schematic diagram of the experiment
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Fig. 2. Ratio of SERS signal intensity of experiment and control
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Fig. 3. Distribution of SERS signal intensity from the aptamer solution to BoNT with concentration of 35.0 nM on the substrate
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Fig. 4. SERS signal intensity distribution from the aptamer solution to BoNT with 17.5 nM concentration on the substrate
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Fig. 5. SERS signal intensity distribution from the aptamer solution to BoNT with 8.8 nM concentration on the substrate
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Fig. 6. SERS signal intensity distribution of experiment and control for BoNT A (2.4 ng/ml) detection using a solution of aptamers with a concentration of 17.5 nM on the substrate
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Fig. 7. Distribution of SERS intensity of experiment and control in the determination of IgGHum (24 ng/ml) using a solution of aptamers to BoNT with a concentration of 17.5 nM on the substrate
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Fig. 8. Distribution of SERS intensity of the experiment and control in the determination of BoNT A (2.4 ng/ml) using a solution of aptamers to RSV with a concentration of 17.5 nM
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