Rapid detection of A-type botulinum toxin using an aptasensor and SERS

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

We described the development of a biosensor for the rapid and sensitive detection of botulinum toxin type A. The sensor is a SERS substrate with an optimized concentration of labeled aptamers, immobilized on its surface. It allows the detection of botulinum toxin type A with a detection limit of 2.4 ng/ml in 1 hour.

Негізгі сөздер

Толық мәтін

Рұқсат жабық

Авторлар туралы

O. Ambartsumyan

Moscow Institute of Physics and Technology

Хат алмасуға жауапты Автор.
Email: ambartsumian.oa@mipt.ru
Ресей, Moscow

A. Brovko

Moscow Institute of Physics and Technology

Email: ambartsumian.oa@mipt.ru
Ресей, Moscow

Әдебиет тізімі

  1. Pirazzini M., Rossetto O., Eleopra R., Montecuccco C. // Pharmacol. Rev. 2017. V. 69. P. 200.
  2. Smith T.J., Hill K.K., Raphael B.H. // Res. Microbiol. 2015. V. 166. P. 290.
  3. Rummel A. // Toxicon. 2015. V. 107. P. 9.
  4. Tian D., Zheng T. // PLoS One. 2014. V. 9. P. 1.
  5. Hill K.K., Smith T.J., Helma C.H. et al. // J. Bacteriol. 2007. V. 189. No. 3. P. 818.
  6. Strotmeier J., Willjes G., Binz T., Rummel A. // FEBS Lett. 2012. V. 586. P. 310.
  7. Kavalali E.T. // Nature. Rev. Neurosci. 2015. V. 16. P. 5.
  8. Kamińska A., Winkler K., Kowalska A. et al. // Sci. Rеports. 2017. V. 7. Art. No. 10656.
  9. Lim C.Y., Granger J.H., Porter M. // Analyt. Chim. Acta X. 2019. V. 1. Art. No. 100002.
  10. Kim K., Choi N., Jeon J.H. et al. // Sensors. 2019. V. 19. Art. No. 4081.
  11. Yoo H., Jo H., Oh S.S. // Mater. Adv. 2020. V. 1. P. 2663.
  12. Frevert J. // Drugs R D. 2010. V. 10. P. 67.

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Әрекет
1. JATS XML
2. Fig. 1. Schematic diagram of the experiment

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3. Fig. 2. Ratio of SERS signal intensity of experiment and control

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4. Fig. 3. Distribution of SERS signal intensity from the aptamer solution to BoNT with concentration of 35.0 nM on the substrate

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5. Fig. 4. SERS signal intensity distribution from the aptamer solution to BoNT with 17.5 nM concentration on the substrate

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6. Fig. 5. SERS signal intensity distribution from the aptamer solution to BoNT with 8.8 nM concentration on the substrate

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7. Fig. 6. SERS signal intensity distribution of experiment and control for BoNT A (2.4 ng/ml) detection using a solution of aptamers with a concentration of 17.5 nM on the substrate

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8. Fig. 7. Distribution of SERS intensity of experiment and control in the determination of IgGHum (24 ng/ml) using a solution of aptamers to BoNT with a concentration of 17.5 nM on the substrate

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9. Fig. 8. Distribution of SERS intensity of the experiment and control in the determination of BoNT A (2.4 ng/ml) using a solution of aptamers to RSV with a concentration of 17.5 nM

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