Antioxidant and phenoloxidase protective systems activity in the gut of Pycnoscelus nigra (Brunner, 1865) roaches on different food substrates

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Abstract

This paper investigates changes in the activity of antioxidant enzymes and the phenoloxidase cascade in the Pycnoscelus nigra (Brunner von Wattenwyl, 1865) roach gut in adaptation to new food substrates. Several different food substrates were chosen. The primary goal of the paper was to examine enzyme activity dynamics of the roaches during their adaptation to new food substrates. Phenoloxidase, catalase and peroxidase activity in roach gut was measured at different points in time. Spectrophotometry was utilized to determine enzyme specific activities via optical density. During the experiment the morphometric parameters of the roaches were measured. Various degrees of enzyme activity were observed depending on the experiment stage and food substrates. Certain patterns of adaptation to new food substrates were shown. The roaches experienced varying degrees of stress reflected in the morphometric parameters of individuals. Understanding the process of insect adaptation to new food substrates may perspectively be useful in combating insect pest dispersal, as well as in insect biotechnology.

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About the authors

A. N. Gladkih

Institute of biochemistry and genetics of Ufa Federal Research Centre of the Russian Academy of Sciences; Bashkir State Medical University

Author for correspondence.
Email: gladkih4leksandar@yandex.ru
Russian Federation, Ufa; Ufa

D. A. Khaliullin

South-Ural Botanical Garden-Institute of Ufa Federal Research Centre of the Russian Academy of Sciences

Email: gladkih4leksandar@yandex.ru
Russian Federation, Ufa

L. R. Gaifullina

Institute of biochemistry and genetics of Ufa Federal Research Centre of the Russian Academy of Sciences

Email: gladkih4leksandar@yandex.ru
Russian Federation, Ufa

E. S. Saltykova

Institute of biochemistry and genetics of Ufa Federal Research Centre of the Russian Academy of Sciences

Email: gladkih4leksandar@yandex.ru
Russian Federation, Ufa

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2. Fig. 1. Phenoloxidase activity: activity values ​​that differ significantly from each other (p < 0.05) are indicated in the figure by Latin letters (a, b, c, d, e). Differences within groups indicated by the same letter are statistically insignificant.

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3. Fig. 2. Catalase activity: Significant differences in activity values ​​that differ significantly from each other (p < 0.05) are indicated in the figure by Latin letters (a, b, c, d). Differences within groups indicated by the same letter are statistically insignificant.

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4. Fig. 3. Peroxidase activity: activity values ​​that differ significantly from each other (p < 0.05) are indicated in the figure by Latin letters (a, b, c, d, e, f, g). Differences within groups indicated by the same letter are statistically insignificant.

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