Desulfosporosinus shakirovi sp. nov., a sulfate-reducing bacterium with ability to petroleum hydrocarbons degradation

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A new sulfate-reducing strain SRJS8T with a high capacity to biodegradate petroleum hydrocarbons has been isolated from the bottom sediments of the northern part of the Sea of Japan. The cells were gram-positive, single-spore-forming motile rods 0.4–0.5 × 2.0–5.0 µm in size. Strain SRJS8T grew at the temperature range from 6°C to 30°C (optimum at 25°C), pH 6.3–7.7 (optimum 7.3), and NaCl concentrations ranging from 0 to 20g/L (optimum 2 g/L). Strain SRJS8T used butanol, glycerol, methanol, ethanol, butyrate, lactate, pyruvate, formate, yeast extract, H2/CO2, crude oil as electron donors and carbon source in the presence of sulfate. SRJS8T used sulfate, sulfite, thiosulphate, elemental sulphur, fumarate and Fe(III) as electron acceptors with lactate presence. The closest relative of the SRJS8T was Desulfosporosinus lacus STP12T with 98.49% similarity. The complete genome of strain SRJS8T (5.43 Mb) was sequenced. DNA–DNA homology of the SRJS8T and D. lacus was 57.4%, and the ANI value was 93.69%. The genomic DNA G + C content was 42.08%. In accordance to the obtained data, SRJS8T is a novel species within the Desulfosporosinus genus, for which the name Desulfosporosinus shakirovi sp. noV. is proposed. The type strain is SRJS8T (= VKM B-3489T, = JCM 39189T).

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A. Eskova

V.I. Il’ichev Pacific Oceanological Institute Far Eastern Branch Russian Academy of Sciences

编辑信件的主要联系方式.
Email: alena-esya@mail.ru
俄罗斯联邦, Vladivostok, 690041

Ya. Ryzhmanova

FRC “Pushchino scientific center of biological research of Russian Academy of Sciences”

Email: alena-esya@mail.ru

Institute of the Biochemistry and Physiology of Microorganisms

俄罗斯联邦, Pushchino, Moscow region, 142290

V. Trubitsyn

FRC “Pushchino scientific center of biological research of Russian Academy of Sciences”

Email: alena-esya@mail.ru

Institute of the Biochemistry and Physiology of Microorganisms

俄罗斯联邦, Pushchino, Moscow region, 142290

N. Polonik

V.I. Il’ichev Pacific Oceanological Institute Far Eastern Branch Russian Academy of Sciences

Email: alena-esya@mail.ru
俄罗斯联邦, Vladivostok, 690041

A. Ponomareva

V.I. Il’ichev Pacific Oceanological Institute Far Eastern Branch Russian Academy of Sciences

Email: alena-esya@mail.ru
俄罗斯联邦, Vladivostok, 690041

V. Shcherbakova

FRC “Pushchino scientific center of biological research of Russian Academy of Sciences”

Email: alena-esya@mail.ru

Institute of the Biochemistry and Physiology of Microorganisms

俄罗斯联邦, Pushchino, Moscow region, 142290

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2. Fig. 1. SEM micrograph of cells of strain SRJS8T.

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3. Fig. 2. Dendrograms of the SBB of the genus Desulfosporosinus, constructed on the basis of the analysis of nucleotide sequences of the 16S rRNA gene, 1340 nt (a); 49 groups of orthologous genes (b).

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4. Fig. 3. Biodegradation of oil hydrocarbons by strains SRJS8T and D. lacus STP12T.

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5. Fig. 4. Phylogenetic dendrogram showing the amino acid sequence assignment of MasD/AssA and BssA of known n-alkane degraders (the substrates used are shown in parentheses) to strains SRJS8T and D. lacus STP12T (other representatives of the genus Desulfosporosinus are not shown). BssA – benzylsuccinate synthase; MasD/AssA – (methyl)alkyl-succinate synthase; PflD/PflE – subunits of pyruvate formate lyase. The scale bar shows 5% of the putative sequence divergence. The amino acid sequence number of the encoded protein is shown in square brackets.

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6. Supplementary Material
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