Volume 103, Nº 2 (2024)
- Ano: 2024
- ##issue.datePublished##: 19.03.2024
- Artigos: 14
- URL: https://rjpbr.com/0016-9900/issue/view/9574
ENVIRONMENTAL HYGIENE
Prospects of the development and harmonization of hygienic standardization taking into account the risk of odour in the ambient air
Resumo
Introduction. The presence of a specific odour in the ambient air in the areas where various enterprises are located with formal compliance with environmental legislation indicates the need to update the methodology of hygienic standardization of odourous substances.
The purpose of the work is to analyze modern methods of the establishment of standards for odourous complex multicomponent mixtures of substances in the atmospheric air and substantiate ways to harmonize them in this field of knowledge.
Materials and methods. The paper systematizes existing approaches to regulating the quality of atmospheric air in areas where odourous emissions sources are located, taking into account certain provisions of regulatory and methodological documents, publications on approaches, and criteria for quantitative sanitary and hygienic assessment of the quality of the environment and public health, as well as the authors’ long-term experience in this field.
Results. The basic principles of hygienic standardization of the permissible content of odorous mixtures of substances in the atmospheric air taking into account their odour are formulated. The substantiation of hygienic standards is carried out with the allocation of substances identified as priority (indicator), based on the results of studying the production technology and the formation of emissions, olfactory properties of the mixture under experimental conditions, with the possibility of monitoring the established standard in atmospheric air by certified instrumental methods of analysis.
Limitations. The limitations of the study are due to the fact that this article does not fully reflect the issues of the development of the entire atmospheric air quality management system taking into account the risk of odour outside the focus of this work, which will be covered in the preparation of the following articles.
Conclusion. The ongoing process of harmonization of domestic and foreign approaches used in hygienic standardization including the field of regulation of atmospheric air pollution by specific odours makes it necessary to significantly expand approaches to the issues of rationing and assessment of environmental pollution by complex multicomponent emissions of odour-source enterprises taking into account modern experience.
Compliance with ethical standards. The study does not require the submission of the conclusion of the biomedical ethics committee or other documents.
Contribution:
Kuzmin S.V. — research concept and design, article editing;
Budarina O.V. — research concept and design, material collection and processing, text writing;
Rakhmanin Yu.A. — text writing, article editing;
Pinigin M.A. — research concept and design, article editing;
Dodina N.S. — collection and processing of the material, editing of the article;
Skovronskaya S.A. — collection and processing of the material.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The study was carried out within the framework of the state task No. 121090800064-5.
Received: December 11, 2023 / Accepted: December 28, 2023 / Published: March 15, 2024



Hygienic efficiency of ultraviolet disinfection of water in centralized drinking and household water supply systems (systematic review)
Resumo
Introduction. Drinking water supplied to the population must be safe in epidemic terms. However, some viruses and parasites are resistant to the disinfecting effect of traditionally used agents for water treatment. The use of UV irradiation ensures the microbiological safety of water, but there are factors affecting its effectiveness.
The purpose of the review was to systematize scientific data on the effectiveness of the use of UV irradiation in relation to drinking water.
Materials and methods. The search for scientific publications was carried out using literature databases MedLine/PubMed, Scopus, and Science Direct. The total number of publications was one thousand six hundred forty-six. The criteria for including the report in the systematic review were: publications in Russian or English with an available full-text version; samples for the study are water from centralized water supply systems or specially prepared microorganisms; any type of research evaluating the effectiveness of a wide range of doses of UV irradiation against microorganisms.
Results of the analysis of publications. A systematic review included 17 reports about the effect of UV irradiation on bacteria and viruses. The subject of several studies was the resistance of protozoan cysts and helminth eggs to different doses of UV irradiation. According to the results presented in the publication, pathogenic viruses and bacteria, giardia cysts possess of the least resistance to UV irradiation (100% inactivation). 4 log-inactivation was established for Mycobacteia. E. coli and coliphages demonstrate a wide range of indicators of the resistance to ultraviolet radiation due to the diversity of the studied strains.
Conclusion. UV disinfection should be used as an additional method in combination with traditional reagents methods of water treatment. It is necessary to study the effect of UV irradiation on helminth eggs and protozoan cysts.
Contribution:
Kirpichenkova E.V. — the concept and design of the study, collection of material, editing;
Dzhikiya I.Z. — collection of material, writing a text, statistical processing;
Kolodina D.V. — collection of material, writing a text, data processing;
Onishchenko G.G. — the concept of the study, editing the final text.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The study had no sponsorship.
Received: January 10, 2024 / Accepted: February 9, 2024 / Published: March 15, 2024



Assessment and certification systems for reagents, materials and technologies used in water treatment in Russia and abroad
Resumo
The article contains an overview of regulatory standing orders devoted to the issues of methodological approaches to assessment and certification systems of reagents, materials, and technologies used in water treatment in Russia and abroad. When conducting a literature search, the following databases were used: Pubmed, Scopus, Web of Science, MedLine, Global Health, RSCI, as well as a search method based on keywords and citations.
Reagents and materials used in water treatment must neither impair the organoleptic properties of water, support the growth of microorganisms, nor have a cytotoxic or mutagenic effect. Their safety assessment includes studies of impurities, chemical and physical properties, maximum doses, behaviour in water, transformation in different environments, migration, and cumulative effects. Reagents, equipment, and materials in many countries are subject to certification by various organizations and specialized laboratories. There are established procedures for their control, including procurement, inspection, handling, storage, and maintenance to ensure quality. At the same time, there is a number of differences: various criteria and standards are used to assess these parameters (from 0.1 MPC to MPC in the hood), and unlike methods. The research methodology, experimental conditions and methods for assessing the results obtained also differ significantly, which can lead to differences in the final results concluding permission and not permission for use in drinking water supply. Standardization of methods and criteria will provide an integrated and comprehensive approach to the selection of reagents and materials in contact with drinking water, eliminate existing contradictions, and help to unite the efforts of many organizations to create an optimal standardization option.
Contribution:
Alekseeva A.V. — concept and design of the study, writing the text, collecting material and processing data, editing, approval of the final version of the article, responsibility for the integrity of all parts of the article;
Rakhmanin Yu.A. — concept and design of the study, editing, approval of the final version of the article, responsibility for the integrity of all parts of the article;
Tyurina I.А. — collection of material and data processing;
Lebed-Sharlevich Ya.I. — collection of material and data processing.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The study had no sponsorship.
Received: December 4, 2023 / Accepted: February 9, 2024 / Published: March 15, 2024



Lost years of life due to the mortality from diseases of the urinary system in the industrial region of Kazakhstan with air pollution
Resumo
Introduction. The relevance of the epidemiological problems of loss of years due to diseases of the kidney and urinary tract is due to the progressive gain in the number of patients in many regions and countries of the World.
Purpose. Estimation of years of life lost due to the mortality from diseases of the urinary system (DUS) in the Pavlodar region, which is one of the industrial regions of Kazakhstan.
Materials and methods. Medical-demographic (mortality rates) and medical-statistical (primary morbidity rates) data was obtained from the Department of Statistics of the Republican Center for Electronic Health Care of the Republic of Kazakhstan (RCEHC RK). The study period was eight years (from 2015 to 2022). The Years of Life Lost (YLL) indicator was calculated according to methodological recommendations. An assessment of the relationships between population health indicators for pathologies of the urinary system (US) with social factors (gross domestic product per capita — GDP), and environmental indicators (ecological and meteorological) as possible causes of adverse events in human health was carried out using correlation and time series analysis.
Results. In the structure of years of life lost due to the mortality from US organs, chronic renal failure (CRF, 55.96–65.85%) led. In the regional center of Pavlodar, there was a peak in years lost in mortality from DUS over 2021 (3.3‰ YLLs), during the COVID-19 pandemic. According to the average annual losses by age groups, in the Pavlodar region, the maximum losses were detected in the elderly (10.5‰ YLLs) and senile (15.8‰ YLLs) age. An excess of the maximum one-time concentrations of pollutants in the atmospheric air above the MPC level with the highest pollution level in 2018 was registered. Correlation analysis showed a high level of correlation between the loss of years of life because of the mortality due to US diseases with atmospheric air pollution in Ekibastuz (r = 0.80), weak — in Pavlodar region (r = 0.28) and low — in Pavlodar (r = 0.13).
Limitations. Of the environmental pollution factors, only atmospheric air pollution factors were studied, which does not reflect all the possible effects of environmental pollution on population health. In the group of reasons with code R, the conclusion “old age” prevailed, which may have led to an inaccurate analysis of the causes of death.
Conclusion. Regional connections between the loss of years of life due to mortality due to US diseases with atmospheric air pollution and temperature in the winter season, as well as newly identified morbidity with atmospheric air pollution, have been identified.
Contribution:
Bibitova Sh.S., Galiakparova Zh.Zh., Zhaksylyk M.A., Dyagilev M.A., Kosach V.S. — collection and processing of material;
Lopuha I.V., Oralova R.N., Sandybayeva A.K., Khashimov Zh.U. — text writing;
Dyussembaeva N.K. — concept and design of the study, approval of the final version of the article;
Rybalkina D.Kh. — editing, responsibility for the integrity of all parts of the article;
Drobchenko Ye.A. — statistical data processing.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgment: the work was carried out within the framework of the project “Setting Target Indicators of Environmental Quality for the Pavlodar Region”.
Received: November 8, 2023 / Revised: Nnovember 20, 2023 / Accepted: January 28, 2024 / Published: March 15, 2024



OCCUPATIONAL HEALTH
Development of the Russian version of the Copenhagen psychosocial questionnaire COPSOQ III and its adaptation in various occupational groups
Resumo
Introduction. The presence of psychosocial risks at work is associated with problems of psycho-emotional and somatic health of workers. Currently in the Russian Federation, there are no validated tools for assessing and managing psychosocial risk that meet international standards.
The purpose of the study. linguistic and cultural adaptation of the Russian version of the Copenhagen psychosocial questionnaire COPSOQ III, including testing of employees in various occupational fields.
Materials and methods. In accordance with international standards and rules of the COPSOQ International Network, there was carried out a procedure for linguistic and cultural adaptation of the Copenhagen Psychosocial Questionnaire which included several stages: direct translation of all questions (146 items) of COPSOQ III from English into Russian; the formation of two independent direct translation options, on the basis of which the experts of the national group formed a preliminary Russian version of COPSOQ III; reverse translation of the preliminary version and harmonization of translations; discussion and formation of national versions of COPSOQ III (short, medium and long); creation of a test medium (standard) version of COPSOQ in Russian; surveying and interviewing employees of institutions of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing for COPSOQ testing; approval of the final version of COPSOQ III in Russian.
Results. In the process of linguistic and cultural adaptation, there was formed the final version of the COPSOQ III questionnaire in Russian, equivalent to the original and corresponding to the ethnolinguistic features of the population. As part of the creation of the Russian-language version of the COPSOQ III questionnaire, it was tested by two hundred ninety employees of institutions of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing in the Moscow region. Based on the results of a survey and interviews with employees, acceptable indicators of external validity and usability of the Russian version of the tool were established.
Limitations. The occupational groups studied are limited to voluntary research participants, the range of problems studied is limited to the questions of the questionnaire, quantitative and qualitative parameters: 290 respondents, departmental affiliation and features of corporate culture: employees of institutions of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing, sociocultural features: subjective perception of the questions and respondents’ doubts about the anonymity and confidentiality of the study.
Conclusion. In accordance with international rules and standards, in the process of linguistic and cultural adaptation, a Russian version of the COPSOQ III questionnaire has been developed. To use the Russian version of COPSOQ III in domestic practice and scientific research, it is necessary to assess its psychometric properties, objectivity, reliability, and specificity as part of the validation procedure.
Compliance with ethical standards. The protocol for linguistic and cultural adaptation of the COPSOQ III questionnaire was approved by local ethics committee of the F.F. Erisman Federal Research Center for Hygiene dated January 10, 2023 N 1-A.
Contribution:
Novikova A.V. — concept and design of the study, collection and processing of material, analysis and interpretation of results, review of literature, preparation of the manuscript project;
Perevezentseva A.S. — concept and design of the study, collection and processing of material, analysis and interpretation of results, review of literature, preparation of the manuscript project;
Shirokov V.A. — concept and design of the study, collection and processing of material, analysis and interpretation of results, review of literature, preparation of the manuscript.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The study had no sponsorship. The study was carried out within the framework of the research project “Development of methodological approaches to substantiate the management of the risks of developing psycho-emotional disorders in workers under conditions of increased labor stress” (applied),
reg. No. PTNI 10230328001561-3.3.10, 20242025.
Received: December 08, 2023 / Revised: December 25, 2023 / Accepted: february 9, 2024 / Published: March 15, 2024



Neurovegetative responses in medical staff of maternity institutions
Resumo
Introduction. Factors of the labour process influence on the adaptive capabilities of the cardiovascular and autonomic nervous systems in workers.
Purpose of the study. The study of the possible relationship between the indicator of adaptation of regulatory systems of heart rate variability and the intensity of the labour process in doctors and nurses in obstetric institutions of the second and third levels.
Materials and methods. The intensity of the labour process was determined by the method of timing. To calculate the adaptation index in the trend in the work shift, the hardware-software complex “Varicard 2.51” was used. Two hundred twenty eight doctors and nurses were examined. The studies were carried out on the basis of obstetric institutions of the second and third levels of the cities of the Central Federal District: Ryazan, Kolomna, Lipetsk, Smolensk. Statistical processing of research results was carried out using standard methods (p < 0.05).
Results. The studies were carried out on the basis of obstetric institutions of the second and third level in some cities of the Central Federal District: Ryazan, Kolomna, Lipetsk, Smolensk. The data obtained in the study of the peculiarities of the regulatory potential in medical workers of various occupational groups made it possible to get an idea of the influence of the factors of the labour process on the adaptive resources, assessed by the indicators of the temporal analysis. The data obtained made it possible to identify a direct correlation between the intensity of the labour process and the developing neurovegetative responses of the organism, which were reflected in the adaptation index in ccupational groups of obstetrician-gynecologists, nurse anesthetists, and midwives of perinatal centers.
Limitations. The studies were carried out in small groups taking into account the trend in the work shift, which opens up the prospect of studying the issue in the age aspect in large groups.
Conclusion. The results of the study can be used to develop evidence-based recommendations for the prevention of occupationally conditioned overstrain of the organism’s adaptive systems.
Compliance with ethical standards. The study was conducted in compliance with the principle of voluntary informed consent of the respondents.
Contribution:
Kotelevets E.P. — collection and processing of material, statistical processing, writing the text;
Kiryushin V.A. — the concept and design of the study, editing.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The study had no sponsorship.
Received: January 18, 2023 / Revised: March 23, 2023 / Accepted: February 9, 2024 / Published: March 15, 2024



Working conditions and health status of school workers (literature review)
Resumo
In recent years, the range of publications concerning the high likelihood of teachers developing syndromes of professional burnout and professional destruction has been increasingly expanding. This trend is almost equally characteristic of most countries over the world: from Great Britain, Germany, and Scandinavia in Europe to China, Japan in Asia and Nigeria in Africa, and, over the last decade, in Russia. In other words, the problem under consideration is global in nature and depends little on the level of economic development of the country and its ethno-geographical characteristics.
Purpose of work. Study foreign and domestic scientific literature directly related to the problems of creating working conditions and their impact on the health in education workers.
The presented review is based on the publications of a number of domestic and foreign studies. The literature search was carried out using the databases Pubmed, Scopus, Web of Science, MedLine, RSCI. A total of forty two publications were analyzed for the period from 2001 to 2022.
A systematic analysis of the world scientific literature was conducted on the management of events aimed at assessing the working conditions and health in teachers. The study examined various aspects related to the provision of medical care to education workers.
After evaluating the results of the study, it became clear that there is some discrepancy between reports in the field of psychology and hygiene. In the first group of works devoted to the psychological aspect, insufficient attention is paid to the study of working conditions and their impact on the health of teachers, despite the widespread prevalence of professional burnout syndrome among the teaching community. At the same time, in the second group of studies devoted to hygienic aspects, the role of psychosocial factors influencing the health of teachers is not sufficiently addressed.
Conclusion. In general, the analysis of the characteristics of working conditions and health of pedagogical workers allows concluding this topic to be relevant and in demand in the scientific world. It also highlights the need for further research and development in this area to improve diagnosis, treatment, and prevention of related diseases.
Contribution:
Stepanov E.G. — research concept and design, data collection and processing, text writing;
Kaibyshev V.T. — data collection and processing, text writing;
Masyagutova L.M. — writing the text, editing;
Sadrtdinova G.R. — writing the text, selection and translation of literature.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The study had no sponsorship.
Received: January 17, 2024 / Accepted: January 9, 2024 / Published: February March 15, 2024



PREVENTIVE TOXICOLOGY AND HYGIENIC STANDARTIZATION
Bioethics and toxicological-hygienic research
Resumo
There are presented and discussed documents regulating the ethical component of working with experimental animals and humans when examining public health, including documents regulating the use of genomic samples in clinical trials of drugs and the study of other factors in the human environment.
Contribution:
Revazova Yu.A. — concept and collection of literary data, writing the text;
Zhukova V.V. — collection of literary data;
Ilyushina N.A. — collection of literary data, writing the text;
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The study had no sponsorship.
Received: January 16, 2024 / Accepted: February 9, 2024 / Published: March 15, 2024



Induction of apoptosis at the molecular genetic level exposed to lead oxide nanoparticles in a chronic animal experiment
Resumo
Introduction. Workers of industrial enterprises and the population living nearby are at risk of lead poisoning. Lead exposure can lead to irreversible negative consequences for the body, including hepatic and renal dysfunction, hematopoietic damage, cognitive dysfunction, and impairment of the genetic apparatus of the human cell. In this regard, it is necessary to study characteristics of the toxic effect of lead oxide nanoparticles (PbO NPs) to determine their health effects and minimize related disorders and diseases.
The purpose of the study was to determine the level of expression of the BAX, BCL-2, P53, GSTM1, GSTP1, and SOD2 genes in various organs of laboratory rats following the exposure to lead oxide nanoparticles.
Materials and methods. Twenty mature female albino Wistar rats were used in a four month experiment with chronic inhalation exposure to PbO NPs, 10 animals per group (exposure and control). The mean concentration of PbO NPs in the inhaled air was 0.215 mg/m3. At the end of the exposure period, organ fragments from the decapitated animals were fixed in liquid nitrogen and subsequently stored in a freezer at –80 °C. Total RNA was isolated from tissues using the ExtractRNA reagent. The expression level was determined by quantitative reverse transcription real-time PCR.
Results. The BAX expression in the liver of rats exposed to PbO NPs for 4 months was by 2.2 times higher than in the control group (p=0.009). We observed a trend towards an increase in the BAX/BCL-2 ratio in hepatocytes indicating apoptotic processes. The P53 expression level was by 1.4 times higher in the olfactory bulb of the exposed rats (p = 0.025) when compared to the controls. No changes were found in the expression levels of antioxidant genes GSTM1, GSTP1, and SOD2.
Limitations. The study was conducted using female Wistar rats with no potential sex differences taken into account.
Conclusion. Chronic inhalation exposure to PbO NPs induces apoptosis in rat liver through the BAX/BCL-2 pathway and rat brain through the regulation of P53.
Compliance with ethical standards. According to the Conclusion of the local Ethics Committee of the Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers keeping, nutrition, care of animals and their removal from the experiment were carried out in accordance with generally accepted requirements, taking into account ARRIVE guidelines. The studies were approved by the Ethics Committee of the Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers (protocol No. 4 of July 12, 2022).
Contribution:
Bereza I.A. — data collection and processing, statistical analysis, draft manuscript preparation, editing;
Shaikhova D.R. — data collection and processing, draft manuscript preparation, editing;
Amromina A.M. — data collection and processing, draft manuscript preparation, editing;
Ryabova Yu.V. — data collection, editing;
Minigalieva I.A. — study conception and design, editing;
Sutunkova M.P. — study conception and design.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The study had no sponsorship.
Received: January 10, 2024 / Accepted: February 9, 2024 / Published: March 15, 2024



Effect of chronic intake of cadmium chloride on the transcriptional activity of metallothionein and zinc transporter genes
Resumo
Introduction. Cadmium chloride is an inorganic compound containing cadmium, a heavy metal that is one of the active environmental pollutants today. Damage to organs in experimental animals due to cadmium poisoning is similar to that in humans. In this work, the activity of metallothionein and zinc transporters genes was studied in a chronic model of cadmium-induced poisoning in experimental animals.
Materials and methods. The experiment was carried out using seventy two individuals of white inbred rats of both sexes, the average weight of which was 215 g. Animals from four groups were injected with a solution of cadmium chloride in four different doses, respectively, individuals of the fifth group, the control group, received an equimolar volume of pure water. The objects of the study were the kidneys and livers of rats, removed after the animals were withdrawn from the experiment. Next, the activity of the Mt1A, Mt2A, Mt3A, Zip1 and Znt1 genes was analyzed in organ samples using real-time PCR.
Results. Significant increases in the expression multiplicity of Mt1A, Mt2A and Mt3A metallothionein genes in the kidneys at different doses of the toxicant were revealed. In liver samples, a decrease in the expression of the Mt2A gene was found in the experimental group exposed to cadmium chloride at a dose of 0.1 mg/kg (p<0.05). For the Znt1 gene in rat liver tissue, there was a statistically significant decrease in expression at a dose of 0.001 mg/kg (p<0.05) and, conversely, an increase at doses of 0.1 and 1 mg/kg (p<0.05) compared to the control group. Analysis of the level of transcripts of the Zip1 gene in the kidneys and liver after 6 months of inoculation with the toxicant in the presented doses did not reveal statistically significant differences between the groups.
Limitations. Laboratory animals of the only biological species were used for the experiment. Four doses of the cadmium salt alone were evaluated.
Conclusion. The results obtained allow concluding that the level of expression of the Mt1A, Mt2A and Mt3A genes in the kidneys can play the role of a diagnostic marker in chronic poisoning with the toxicant under study.
Compliance with ethical standards. Date of the meeting of the bioethical commission of the Federal Budgetary Institution “Ufa Research Institute of Occupational Medicine and Human Ecology” 12/11/2023 No. 01-12. Throughout the study, the animals were kept under standard conditions with 12 hours of artificial lighting during the day, a relatively constant level of humidity (30–70%) and an air temperature of 20–25 °C. Manipulations with all animals were carried out strictly in compliance with the rules prescribed in basic regulatory documents, including the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Strasbourg, 1986) and the Declaration of Helsinki on the Humane Treatment of Animals.
Contribution:
Gizatullina A.A. — concept and design of the study, collection and processing of material, statistical processing, text writing;
Valova Y.V. — collection and processing of material, statistical processing;
Khusnutdinova N.Yu., Smolyankin D.O., Karimov D.D. — collection and processing of material;
Karimov D.O. — concept and design of the study, statistical processing;
Muhammadieva G.F. — editing;
Repina E.F. — concept and design of the study.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. Industry research program of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare for 2021–2025. clause 6.1.9. "Experimental substantiation of highly sensitive markers of the effects of toxic metals on the body and development of preventive measures".
Received: December 26, 2023 / Revised: January 29, 2024 / Accepted: February 29, 2024 / Published: March 15, 2024



Neurotoxic effects of cadmium chloride exposure combined with physical activity and protective effect of bioprophylactic agents
Resumo
Introduction. Chemical compounds possessing of a neurotropic effect are extremely widespread in industry, which makes the problem of neurotoxicity relevant for occupational medicine. Since industrial workers are often exposed to a combination of both physical and chemical work-related risk factors, a complex adverse health effect of the latter should be considered.
Our objective was to study neurotoxic effects of exposure to cadmium chloride combined with physical activity in a subchronic experiment on rats and assess the efficacy of a biological prophylactic complex.
Material and methods. For six weeks, 0.77 mg/kg b.w. of cadmium chloride was intraperineally instilled to outbred male albino rats thrice a week. Five times a week, the rodents were forced to run for 10 minutes at a speed of 25 m/min. During the entire exposure period, half of the animals received a specially developed bioprophylactic complex consisting of pectin, monosodium glutamate, and a multivitamin/multimineral supplement with feed and drink.
Results. In combination with physical activity, cadmium exposure caused depression, anxiety, low exploratory behaviour, and spatial memory disturbances. The developed bioprophylactic complex helped mitigate toxic effects of cadmium aggravated with intense physical activity and improve the general condition of the rodents.
Limitations. The experiment was limited to examining the behaviour of male rats following subchronic exposure to a single dose of cadmium.
Conclusions. Subchronic exposure to cadmium combined with physical activity can induce certain neurotoxic effects. Administration of the specially developed complex of biological protectors has shown to attenuate or minimize these effects. Similar measures can be taken to diminish risks of adverse health consequences of the factors studied.
Compliance with ethical standards. The study was carried out in accordance with ethical principles for the treatment of animals adopted by the European Convention for the Protection of Vertebrate Animals Used for Experiments and Other Scientific Purposes (ETS 123). The study protocol was approved by the Local Independent Ethics Committee of the Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers (protocol No. 4 of September 30, 2022).
Contribution:
Ryabova Yu.V. — study conception and design, draft manuscript preparation;
Shabardina L.V. — data collection and analysis, draft manuscript preparation;
Keskevich A.A. — data collection and analysis, draft manuscript preparation;
Minigalieva I.A. — study conception and design, draft manuscript preparation;
Sutunkova M.P. — study conception and design;
Butakova I.V. — data collection and analysis;
Bateneva V.A. — data collection and analysis;
Privalova L.I. — study conception and design, scientific editing.
All authors are responsible for the integrity of all parts of the manuscript and approval of its final version.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The study had no sponsorship.
Received: February 5, 2024 / Accepted: February 9, 2024 / Published: March 15, 2024



HEALTH RISK ASSESSMENT
Assessment of the epidemiological health risk of foundry contamination of small town soils
Resumo
Introduction. The paper presents the results of the assessment of the risk to public health and the level of soil contamination with heavy metals and arsenic in a small town with a foundry.
Materials and methods. The objects of the study are the morbidity of the population of the Melenkovsky district of the Vladimir region and the soil cover of the city of Melenki. The assessment of the probable risk of morbidity is based on the determination of epidemiological risk indicators, the obtained values of which were converted into values normalized by the marginal error of the background level (Δ). Soil samples were taken in the zone of industrial enterprises, highways, residential private sector, landscape, and recreational areas. The soil cover was examined by X-ray fluorescence method for the evaluationof the content of heavy metals and arsenic.
Results. The study conducted to assess the probable risk allowed establishing in the Melenkovsky district, relative to the background regional values, children to have a very high risk for nine classes of diseases, adults to have a very high risk for 6 classes of diseases. There has been established the contamination of the soil of the city of Melenka with heavy metals and arsenic to be probably due to the specifics and duration of operation of the oldest enterprise in the city ‒ the foundry and mechanical plant. In the central part of the city, there have been identified local areas posing high health risks, which determines the need to manage permanent environmental and hygienic monitoring here. The greatest danger to public health is arsenic, whose concentrations in almost all sampling points exceed sanitary and hygienic standards.
The limitations of the study are related to one-time sampling and a small number of reference sites, which limits the possibilities of a broader interpretation of the data obtained.
Conclusion. It has been established that a city enterprise operating for a long time, due to soil contamination of the adjacent territory, can bear high risks to public health even during prolonged downtime or termination of its main activity. The chemical contamination of the soil in the territory adjacent to the foundry and mechanical plant, which is extremely dangerous in terms of the total indicator, requires special environmental measures to remove and further neutralize chemical elements. To reduce soil pollution in the city, there are required modernization of industrial enterprises and the creation of expanded sanitary protection zones around them.
Compliance with ethical standards. The study does not require the conclusion of the Biomedical ethics committee.
Contribution:
Trifonova T.A. — concept and design of the study;
Martsev A.A. — research concept and design, data processing, text writing, final design;
Selivanov O.G. — data processing, text writing;
Kurbatov Yu.N. — laboratory research;
Rostunov A.O. — sampling and sample preparation.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The study had no sponsorship.
Received: June 26, 2023 / Revised: October 24, 2023 / Accepted: February 9, 2024 / Published: March 15, 2024



ANNIVERSARY DATES
To 85th Anniversary since birthday of Lena Mirzaevna Karamova
Resumo
Лена Мирзаевна Карамова — доктор медицинских наук, профессор, член-корреспондент Академии наук Республики Башкортостан, академик Европейской академии естественных наук и Российской Академии Естествознания, Заслуженный врач Республики Башкортостан и Российской Федерации, Заслуженный деятель науки Республики Башкортостан, Почётный работник Госсанэпидслужбы России (2012 г.).



To 55th Anniversary of Valery Ivanovich Popov
Resumo
25 февраля 2024 г. исполнилось 55 лет со дня рождения и 31 год научно-педагогической и общественной деятельности Валерия Ивановича Попова — заведующего кафедрой общей гигиены ФГБОУ ВО «Воронежский государственный медицинский университет им. Н.Н. Бурденко» Минздрава России.


