Creation of a database of historical control of hematological indices in rat blood to interpret the results of sanitary and toxicological studies of active substances of pesticides

Introduction. Historical control data provide an understanding of the background variability (spontaneous changes) of the studied indices in the same species and line of animals, under similar conditions in the same laboratory, when assessing the toxic effects of repeated (manifold) administration of active substances of pesticides.

The aim of the study was to create a database of historical control of hematological indices in rat blood used in chronic (twelve months) studies of active substances of pesticides of various chemical structures.

Materials and methods. In accordance with the planned goal, a database of hematological blood indicces from control animals used in chronic (12 months) sanitary and toxicological studies of active substances of pesticides of various chemical structures has been created. The database contains information on 13 hematological indices in rat blood taken from control animals in dynamics after 1, 3, 6 and 12 months from the start of the study for the period 2017–2022. Studies of the active substances of pesticides were carried out in the biological testing laboratory of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing according to the manual P 1.2.3156–13.

Results. The results of the conducted studies indicate that in dynamics after 1, 3, 6 and 12 months of the study, a statistically significant increase in hemoglobin levels was detected in the blood of male rats. With the age, the animals showed a tendency to increase the concentration of red blood cells and platelets.

Limitations. This article publishes a database of historical control of hematological parameters in rat blood, data on biochemical indices of blood serum and physiological indices in male rats in similar studies will be presented in the following publications.

Conclusion. The results obtained will be used to substantiate the maximum inactive doses (NOEL), permissible daily doses (DSD), hygienic standards of the studied compounds in food and environmental objects.

Compliance with ethical standards. The research was approved by the Ethics Committee of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (minutes of the meeting No. 10 dated 17/11/2016)

Contribution:
Rakitskiy V.N.,  Kuzmin S.V. — concept and design of the study, writing text, editing;
Epishina T.M. — conducting research, collecting and statistical processing of the received material, writing text, compiling a list of references;
Chkhvirkia E.G. — concept and design, text editing.
All co-authors — approval of the final version of the article, responsibility for the integrity of all parts of the article.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The study had no sponsorship.

Received: September 4, 2024 / Accepted: October 2, 2024 / Published: November 19, 2024