Changes in microbial communities in primary soil and ground under the anthropogenic influence on the territory around Antarctic station "Mirny"
- Autores: Kirtsideli I.Y.1, Teshebaev S.B.2, Vlasov D.Y.1,3, Novozhilov Y.K.1, Abakumov E.V.3, Barantsevich E.P.4, Krylenkov V.A.3, Zelenskaya M.S.3
-
Afiliações:
- Komarov Botanical Institute of Russian Academy of Sciences
- Arctic and Antarctic Research Institute
- Saint-Petersburg State University
- Almazov Northwestern Federal Medical Research Center
- Edição: Volume 96, Nº 10 (2017)
- Páginas: 949-955
- Seção: ENVIRONMENTAL HYGIENE
- ##submission.datePublished##: 21.10.2020
- URL: https://rjpbr.com/0016-9900/article/view/640707
- DOI: https://doi.org/10.47470/0016-9900-2017-96-10-949-955
- ID: 640707
Citar
Texto integral
Resumo
Antarctic station “Mirny” was the first Russian (Soviet) polar station in Antarctica. The anthropogenic impact on the primary ground and soil in the area around of this polar station took place more than 60 years. This is reflected in both the chemical composition of the soil and microbial communities. The article shows multiple increasing of the number of bacteria and microscopic fungi in anthropogenic contaminated soils. Contamination of soil leads to changes of the structure of the microbial community. An increasing proportion of mesophilic bacteria isolates (90%) was shown. The increasing share spores and reduced the proportion of microscopic fungi mycelia were observed. Statistical analysis showed that we have identified almost all of the expected species that live in anthropogenic substrates (42 species), as well as in the control soils (17 species). But we identified only a part of fungal diversity in contaminated soils (32 species). The increase in the number of fungal species in the area of the polar station “Mirny” was proved to be connected with the human activity and introduction of new species. Native species of microscopic fungi were found to be able to colonize new materials previously unavailable to them. They also can be agents of active biodegradation of anthropogenic materials. Among fungal species isolated from anthropogenic contaminated soil and anthropogenic substrates more than 80% can be attributed to opportunistic organisms. Therefore the total number of microorganisms (CFU), the structure of the microbial complexes, the species composition of communities, indicator species, morphology and volume of biomass can be considered as an index of changes in soil microbial complexes and primary soils
Palavras-chave
Sobre autores
Irina Kirtsideli
Komarov Botanical Institute of Russian Academy of Sciences
Autor responsável pela correspondência
Email: microfungi@mail.ru
ORCID ID: 0000-0002-4736-2485
MD, PhD, DSci., leading researcher of the mycology department of Komarov Botanical Institute of Russian Academy of Science, St. Petersburg, 197376, Russian Federation.
e-mail: microfungi@mail.ru
RússiaSh. Teshebaev
Arctic and Antarctic Research Institute
Email: noemail@neicon.ru
ORCID ID: 0000-0002-7530-0150
Rússia
D. Vlasov
Komarov Botanical Institute of Russian Academy of Sciences; Saint-Petersburg State University
Email: noemail@neicon.ru
ORCID ID: 0000-0002-0455-1462
Rússia
Yu. Novozhilov
Komarov Botanical Institute of Russian Academy of Sciences
Email: noemail@neicon.ru
ORCID ID: 0000-0001-8875-2263
Rússia
E. Abakumov
Saint-Petersburg State University
Email: noemail@neicon.ru
ORCID ID: 0000-0002-5248-9018
Rússia
E. Barantsevich
Almazov Northwestern Federal Medical Research Center
Email: noemail@neicon.ru
ORCID ID: 0000-0002-4800-3345
Rússia
V. Krylenkov
Saint-Petersburg State University
Email: noemail@neicon.ru
ORCID ID: 0000-0002-7103-3091
Rússia
M. Zelenskaya
Saint-Petersburg State University
Email: noemail@neicon.ru
ORCID ID: 0000-0003-3588-8583
Rússia
Bibliografia
- Gilichinsky D. Permafrost as a microbial habitat. In: Bitton G. Encyclopaedia of Environmental Microbiology. New York: Wiley; 2002: 932–56.
- Lopatina A., Krylenkov V., Severinov K. Activity and bacterial diversity of snow around Russian Antarctic stations. Res. Microbiol. 2013; 164(9): 949–58.
- Waterhouse E.J. Ross Sea Region: A state of the environment report for the Ross Sea region of Antarctica. Christchurch: New Zealand Antarctic Institute; 2001.
- Bargagli R. Environmental contamination in Antarctic ecosystems. Sci. Total Environ. 2008; 400: 212–26.
- Ott S. The diversity of colonising diasporas at an Antarctic inland site implication for ecosystem development. In: Polar Research – Arctic and Antarctic perspectives in the International Polar Year. St. Petersburg; 2008.
- Arenz B.E., Held B.W., Jurgens J.A., Farrell R.L., Blanchette R.A. Fungal diversity in soils and historic wood from the Ross Sea Region of Antarctica. Soil Biol. Biochem. 2006; 38: 3057–64.
- Arenz B.E., Held B.W., Jurgens J.A., Blanchette R.A. Fungal colonization of exotic substrates in Antarctica. Fungal Divers. 2011; 49(1): 13–22.
- Osyczka P., Mleczko P., Karasinki D., Chlebicki A. Timber transported to Antarctica: a potential and undesirable carrier for alien fungi and insects. Biol Invasions. 2012; 14: 15–20.
- Zelenskaya M.S., Kirtsideli I.Yu., Vlasov D.Yu., Krylenkov V.A., Sokolov V.T. Micromycetes – Biodestructors in ecosystems of the Arctic. Problemy regional’noy ekologii. 2013; (5): 135–41. (in Russian)
- Kirtsideli I.Yu., Vlasov D.Yu., Krylenkov V.A., Sokolov V.T. Aeromikota in locations of the Russian Arctic stations in the water areas of the White, Barents and Kara Seas. Mikologiya i fitopatologiya. 2011; 45(3): 228–39. (in Russian)
- Kirtsideli I.Yu., Vlasov D.Yu., Barantsevich E.P., Krylenkov V.A., Sokolov V.T. Distribution of terrigenous microfungi in arctic seas. Mikologiya i fitopatologiya. 2012; 46(5): 306–10. (in Russian)
- Kirtsideli I.Yu., Vlasov D.Yu., Barantsevich E.P., Krylenkov V.A., Sokolov V.T. Complexes of microscopic fungi in soils of polar islands Izvestiy TSIK (Kara Sea). Mikologiya i fitopatologiya. 2014; 48(6): 365–71. (in Russian)
- Kirtsideli I.Yu., Abakumov E.V., Teshebaev Sh.B., Zelenskaya M.S., Vlasov D.Yu., Krylenkov V.A., et al. Microbial communities in regions of the arctic settlements. Gigiena i sanitariya. 2016; 95 (10): 923–9. (in Russian)
- Abakumov E.V., Lodygin E.D., Gabov D.A., Krylenkov V.A. Polycyclic aromatic hydrocarbons content in Antarctica soils as exemplified by the Russian Polar stations. Gigiena i sanitariya. 2014; 93(1): 30–5. (in Russian)
- Zvyagintsev D.V. Methods of Soil Microbiology and Biochemistry [Metody pochvennoy mikrobiologii i biokhimii]. Moscow; 1991. (in Russian)
- Pestova N.E., Barantsevich E.P., Rybkova N.S., Kozlova N.S., Barantsevich N.E. Study of effectiveness of sequences of fragment of 16S RRNA gene in identification of microorganisms. Profilakticheskaya i klinicheskaya meditsina. 2011; (4): 54–5. (In Russian).
- Colwell R.K., Chao A., Gotelli N.J., Lin S.Y., Mao C.X., Chazdon R.L., et al. Models and estimators linking individual-based and sample based rarefaction, extrapolation and comparison of assemblages. J. Plant. Ecology. 2012; 5(1): 3–21.
- Shitikov V.K., Zinchenko T.D., Rozenberg G.S. Macroecology of River Communities: Concepts, Methods, Models [Makroekologiya rechnykh soobshchestv: kontseptsii, metody, modeli]. Tol’yatti; 2011. (in Russian).
- Orlov D.S., Grishina L.A. The Practice of Chemistry of Humus [Praktikum po khimii gumusa]. Moscow; 1981. (in Russian)
- U.S. Environmental Protection Agency. Method 8310: Polynuclear Aromatic Hydrocarbons, 1986.
- U.S. Environmental Protection Agency. Evaluation and estimation of potential carcinogenic risks of polynuclear aromatic hydrocarbons: carcinogen assessment group. Washington DC: Office of Health and Environmental Assessment; 1995.
- U.S. Environmental Protection Agency. Method 3550b: Ultrasonic extraction. Washington DC: Office of Health and Environmental Assessment; 1996.
- U.S. Environmental Protection Agency. Method 3630: Silica gel cleanup. Washington DC: Office of Health and Environmental Assessment; 1996.
- ОSТ 10-259–2000. Soil. X-ray fluorescence determination of the gross content of heavy metals. Moscow; 2000. (in Russian)
- Sanitary rules SP 1.3.2322–08. Security work with microorganisms of III–IV pathogenicity groups (hazard) and agents of parasitic infections. Moscow; 2008. (in Russian)
Arquivos suplementares
