Study the pathological impact of blastocyst invasion and fungi of Candida spp. overlay in the system of associative symbiosis in the human intestine

Introduction. The processes of socio-economic restructuring in recent decades, taking place in our country, have led to a reduction in the volume and deterioration in the quality of measures to protect various components of the human environment from contamination by parasitosis pathogens. The number of diagnostic examinations of the population to identify patients with parasitic diseases has decreased. Every year, the interest in this problem of the medical and scientific community increases in the scientific literature of both foreign and Russian authors. There are more and more new prerequisites for studying the interaction of the intestinal microflora, parasites, the immune response inflammatory processes of the human body. Today, the role of parasitic intestinal protozoa Blastocystis spp., and in some cases aggravated by the addition of opportunistic fungi of the genus Candida spp., is increasingly being discussed in human pathology. 

The aim of the study was to assess the possible relationship between the presence of blastocyst invasion in the examined group of “practically healthy people” with various abnormalities in the gastrointestinal tract (diarrhoea, constipation, single or repeated pain, etc.), immunopathological processes (skin rashes, intolerance to any food, etc.), as well as the addition of opportunistic fungal microflora of the genus Candida spp.

Materials and methods. A complex of parasitological and additional research methods was used, including a culture method for isolating fungi of the Candida spp. A complete analysis of the personal data received from the analyzed group was carried out.

Results. The results of studies of the intestine’s clinical material (faeces) and the analysis of personal data in the surveyed “conditionally healthy” group of respondents confirmed our assumption about the pathological effect of blastocyst invasion on the conditionally healthy intestinal microflora and, accordingly, human health.

Limitations. The limitations of this study make it possible to apply the findings to the health of the adult, non-old, able-bodied population and, at the same time, take into account regional characteristics in the development of mixed infections of parasitic and fungal genesis in humans. In this regard, we consider it possible to apply the findings to all regions with similar living conditions (urban lifestyle, cold and temperate climate, etc.)

Conclusions. It is necessary to continue conducting further observations and research of various groups (individuals) in various regions of the Russian Federation, participating in the development of educational and practical algorithms for laboratory diagnostics of parasitoses and mycoses for specialists in clinical laboratories.

Contribution:

Aslanova M.M. — the concept and design of the study, collection of material and literature data, statistical analysis, writing a text, editing;

Gololobova T.V. — the concept and design of the study, writing а text;

Maniya T.R. — collection of material and literature data, statistical analysis;

Kurbatova I.V. — collection of literature data;

Rakitina D.V., Starodubova N.Yu. — collection of material and data processing, statistical processing;

Zagainova A.V., Yudin S.M. — editing.

All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest. 

Acknowledgement. The research was carried out within the framework of the research work “Development of technologies for cryopreservation and archiving of biological samples of human microecological resources (code Cryobank)”.

Received: September 17, 2021 / Accepted: November 25, 2021 / Published: February 09, 2022