Immune and neurohumoral profile of the children population living in the conditions of exposure to benzo(a)pyrene

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Introduction. Benzo(a)pyrene induces disorders of immune and neurohumoral regulation that are aggravated by the influence of unfavourable climatic and geographical factors in the Far North. In this regard, studies of the features of the immune and neurohumoral profile are of particular relevance for the identification of markers of early health disorders of the population of industrially developed circumpolar territories (using the example of benzo(a)pyrene).

Materials and methods. Five hundred 3–6 year children living in the circumpolar territory of Eastern Siberia were examined. Observation group consisted of 352 children living in conditions of exposure to benzo(a)pyrene. Comparison group included 148 children residing at a relatively clean territory. Determination of the content of benzo(a)pyrene in the blood was carried out by HPLC. Phenotyping of CD3+-, CD19+-, CD3+CD95+-, Annexin V-FITC+7AAD– and Annexin V-FITC+7AAD+-lymphocytes was performed by flow cytofluorometry. The level of IgA, IgM, IgG was determined by Mancini radial immunodiffusion, the IgG content to benzo(a)pyrene was determined by allergosorbent testing. The content of acetylcholine, serotonin, and neurotropin-3 was determined by ELISA.

Results. Children living in the industrially developed circumpolar territory of Eastern Siberia have an increased level of blood contamination with benzo(a)pyrene (p<0.05). The immune profile is characterized by inhibition of cellular (CD3+ deficiency) and humoral immunity (decreased IgA, IgM, IgG with an excess of CD19+). Violations of apoptosis (decrease in Annexin V-FITC+7AAD–, Annexin V-FITC+7AAD+-lymphocytes; increase in Bcl-2 and CD95+) were revealed (p<0.05). An increased IgG to benz(a)pyrene content were found (p<0.05). The neurohumoral profile of the examined children is characterized by hyperexpression of serotonin with acetylcholine and neurotropin-3 deficiency (p<0.05).

Limitations. The results of the study are intended for specialists in the field of hygiene, immunology and allergology.

Conclusion. Revealed features of the immune status (deficiency of CD3+, Annexin V-FITC+7AAD–, and Annexin V-FITC+7AAD+-lymphocytes IgA, IgM, IgG, excess CD19+, CD95+, Bcl-2, IgG to benzo(a)pyrene), associated with changes in sympathetic-parasympathetic balance (excess serotonin, deficiency of acetylcholine, neurotropin-3) characterize the features of the immune and neurohumoral profile in the children population of the circumpolar territories of Eastern Siberia under the conditions of exposure to benzo(a)pyrene.

Compliance with ethical standards. The study was carried out in compliance with the ethical requirements of the Helsinki Declaration of the WMA 2000 and the Protocol of the Council of Europe Convention on Human Rights and Biomedicine 1999. The study was approved by the LEC of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies of the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being (Protocol No. 23 of 12/20/2021).

Patient consent. Each participant of the study (or his/her legal representative) gave informed voluntary written consent to participate in the study and publish personal medical information in an impersonal form in the journal "Gigiena i Sanitariya (Hygiene and Sanitation, Russian journal)".

Contribution:
Nikonoshina N.A. — collecting and processing material, statistical processing, writing, and editing text;
Dolgikh O.V. — concept and design of research, writing and editing of text.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest. 

Acknowledgement. The study had no sponsorship

Received: October 27, 2022 / Accepted: December 8, 2022 / Published: January 12, 2023

作者简介

Natalya Nikonoshina

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies” of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

编辑信件的主要联系方式.
Email: nat08.11@yandex.ru
ORCID iD: 0000-0001-7271-9477

Postgraduate student at the Laboratory of immunology and allergology, post-graduate student of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies” of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Perm, 614045, Russian Federation.

e-mail: nat08.11@yandex.ru

俄罗斯联邦

Oleg Dolgikh

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies” of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Email: noemail@neicon.ru
ORCID iD: 0000-0003-4860-3145
俄罗斯联邦

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