Evaluation of the impact of industrial single-walled and multi-walled carbon nanotubes on human respiratory tract epithelial cells

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Abstract

Introduction. In the present study, a comparative assessment of the toxic effects of industrial single-walled and multi-walled carbon nanotubes (SWCNT and MWCNT) at doses corresponding to industrial exposures on BEAS-2B and A549 cells was carried out.

Materials and methods. The size distribution of SWCNT and MWCNT agglomerates in dispersions was estimated by dynamic light scattering and transmission electron microscopy. Cytotoxicity was assessed using a MTS test and LDH assay. The interaction of CNTs with cells was visualized using dark-field and transmission electron microscopy.

Results. Cytotoxic effects of pristine SWCNT and MWCNT in concentrations of 50–200 μg/ml and purified SWCNT in the range of 25–200 μg/ml were found in BEAS-2B cells. SWCNT and MWCNT were found to penetrate into the cytoplasm of both BEAS-2B and A549 cells, while MWCNT are more often revealed in the intracellular content as vacuolized clusters, and single SWCNT and agglomerates are visualized in the cytoplasm without a tendency to vacuolization.

Limitations. CNT were introduced into cells in the form of dispersions, where both single nanotubes and their agglomerates were found. The calculation of CNT concentrations for introduction into cells was based on computer simulation.

Conclusion. Further study of the mechanisms of cytotoxic and genotoxic effects of different types of carbon nanotubes (CNT) may contribute to the identification of MWCNT and SWCNT specific effects on the cells of the respiratory system to develop methodological approaches to the safe use of CNT.

Compliance with ethical standards. The study does not require submission of the opinion of the biomedical ethics committee or other documents.

Contribution:
 Gabidinova G.F. — literature review on the topic of research, cell cultivation, tests (LDH) on cells, statistical data processing, preparation of pictures, generalization of the results;
Timerbulatova G.A. — literature review on the topic of research, cell cultivation, tests (MTS) on cells, preparation of pictures, generalization of the results;
Daminova A.G. — transmission electron microscopy, suspension morphometry, generalization of the obtained results;
Galyaltdinov Sh.F. — preparation of suspensions of materials for introduction into cells;
Dimiev A.M. — development of methods for preparing suspensions of materials for introduction into cells;
Kryuchkova M.A. — visualization of nanomaterials in cells (dark field microscopy);
Fakhrullin R.F. — visualization of nanomaterials in cells (dark field microscopy);
Fatkhutdinova L.M. — material analysis; editing; preparing an article for publication.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version. 

Conflict of interest. The authors declare no conflict of interest.

Acknowledgment. The study was supported by the Russian Science Foundation grant № 22-25-00512, https://rscf.ru/project/22-25-00512/

Received: October 27, 2022 / Accepted: December 8, 2022 / Published: January 12, 2023

About the authors

Gulnaz F. Gabidinova

Kazan State Medical University

Author for correspondence.
Email: noemail@neicon.ru
ORCID iD: 0000-0003-2616-5017
Russian Federation

Gyuzel A. Timerbulatova

Kazan State Medical University; Center of Hygiene and Epidemiology in the Republic of Tatarstan

Email: noemail@neicon.ru
ORCID iD: 0000-0002-2479-2474
Russian Federation

Amina G. Daminova

Kazan Federal University

Email: noemail@neicon.ru
ORCID iD: 0000-0002-7672-4430
Russian Federation

Shamil F. Galyaltdinov

Kazan Federal University

Email: noemail@neicon.ru
ORCID iD: 0000-0002-9494-5288
Russian Federation

Ayrat M. Dimiev

Kazan Federal University

Email: noemail@neicon.ru
ORCID iD: 0000-0001-7497-1211
Russian Federation

Marina A. Kryuchkova

Kazan Federal University

Email: noemail@neicon.ru
ORCID iD: 0000-0002-6946-0553
Russian Federation

Rawil F. Fakhrullin

Kazan Federal University

Email: noemail@neicon.ru
ORCID iD: 0000-0003-2015-7649
Russian Federation

Liliya M. Fatkhutdinova

Kazan State Medical University

Email: liliya.fatkhutdinova@kazangmu.ru
ORCID iD: 0000-0001-9506-563X

MD, PhD, head of the Department of Hygiene and Occupational Medicine, Kazan, 420012, Russian Federation.

e-mail: liliya.fatkhutdinova@kazangmu.ru

Russian Federation

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Copyright (c) 2023 Gabidinova G.F., Timerbulatova G.A., Daminova A.G., Galyaltdinov S.F., Dimiev A.M., Kryuchkova M.A., Fakhrullin R.F., Fatkhutdinova L.M.


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