Using human cell culture to assess the toxicity of water(literature review)

Мұқаба

Дәйексөз келтіру

Толық мәтін

Аннотация

The problem of water sources pollution, connected with increasing anthropogenic charge is attracting a lot of attention nowadays. Most of hygienic evaluation methods of water objects are based on physicochemical analysis of water samples. These methods can’t be considered as consistent in determination of full range of pollutants. Sanitary chemical analysis of water environment, coupled with biological testing seems to be more informative. One of the most prospective research trends nowadays is using human cell lines as test objects. During the preparation of this review, there were used following database sources: Scopus, Web of Science, PubMed, RISC. As a conclusion of performed sources analysis, we can point at high sensitivity of cell lines, extracted from human digestive (Caco-2, HepG2) and excretory systems (HEK-203) to the influence of pollutants taken from different water sources. The data obtained by the authors indicate both a cytopathic effect and a change in the cytochemical and cytomorphological characteristics of cell cultures under the influence of pollutants in water. The use of human cell cultures as test objects in water biotesting is an urgent direction in the study of water supply sources for drinking and household needs of the population without preliminary purification. The use of human cell cultures in the biotesting of water makes it possible to give not only a toxicological characteristic of water samples, but also to assess the possibility of developing an undesirable effect associated with the ingress of pollutants into the internal environment of the body.

Contribution:
Mamonova I.A. — concept and design of the study, writing the text, responsibility for the integrity of all parts of the article;
Kosheleva I.S. — collection and processing of material, writing text, editing;
Shirokov A.A. — writing the text, editing;
Gusev Yu.S. — concept and design of the study;
Mikerov A.N. — approval of the final version of the article, responsibility for the integrity of all parts of the article.

Gratitude. We thank research center «Symbiosis» and immunochemistry laboratory IBPPM RAS for their providing advisory assistance in the selection and analysis of literature data performing research within the framework of the project GR No. 121031100266-3.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgments. The study had no sponsorship. 

Received: March 22, 2023 / Accepted: May 31, 2023 / Published: June 20, 2023

Авторлар туралы

Irina Mamonova

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies; Saratov State Medical University named after V.I. Razumovsky

Хат алмасуға жауапты Автор.
Email: mamonova.83@rambler.ru
ORCID iD: 0000-0003-3941-4334

MD, Ph.D., researcher of Laboratory of chemical-biological monitoring of water quality of the Saratov Medical Scientific Centre of Hygiene of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Saratov, 410022, Russian Federation.

e-mail: mamonova.83@rambler.ru

Ресей

Irina Kosheleva

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Email: noemail@neicon.ru
ORCID iD: 0000-0003-1992-5305
Ресей

Aleksandr Shirokov

Saratov State Medical University named after V.I. Razumovsky; N.G. Chernyshevsky Saratov National Research State University; Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)

Email: noemail@neicon.ru
ORCID iD: 0000-0003-4321-735X
Ресей

Yurij Gusev

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies; N.G. Chernyshevsky Saratov National Research State University; Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)

Email: noemail@neicon.ru
ORCID iD: 0000-0001-7379-484X
Ресей

Anatoly Mikerov

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies; Saratov State Medical University named after V.I. Razumovsky

Email: noemail@neicon.ru
ORCID iD: 0000-0002-0670-7918
Ресей

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© Mamonova I.A., Kosheleva I.S., Shirokov A.A., Gusev Y.S., Mikerov A.N., 2024


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