Effects of genome instability under irradiation in different CT scanning modes. Results of ex vivo pilot cohort study

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Abstract

Introduction. Medical radiation is one of the leading sources of public exposure in the world. In recent decades, the total number of X-ray diagnostic procedures has increased significantly, and with the increase in the volume of computed tomography (CT), a significant gain in the total cumulative radiation dose is also associated.

The aim of the work is to compare the genotoxic effects of irradiation of human blood lymphocytes using various CT protocols.

Materials and methods. Among patients of different genders and ages who sought for a preventive examination, nine practically healthy volunteers (donors) who signed an informed consent, were randomly selected to participate in the ex vivo experiment. 4 venous blood samples from each donor were irradiated on various CT protocols (0.82–11.8 mSv) in an anthropometric phantom of the human chest. Aliquots of each sample were cultured under conditions of cytokinesis block, recorded and analyzed according to the protocol of cytomic analysis in a micronucleus test.

Results. Irradiation of blood samples in the Ultra-NDCT mode (0.82 mSv) revealed a peak in the proliferation of rapidly dividing cells and the frequency of genetic damage in them, and also demonstrated a high probability of the formation and consolidation of genetic damage in generations of dividing cells. This indicates increased genotoxicity and, most likely, immunotropicity of the studied irradiation mode.

Limitation of the study is the inadmissibility of involving a person in an experiment or using biomaterials without obtaining his consent, as well as the inadmissibility of causing physical harm or harm to human honor and dignity during the experiment

Conclusion. When choosing CT modes, it is necessary to take into account not only the levels of effective doses, but also the possibility of developing the effects of genome instability. However, this approach requires additional genotoxic studies of CT protocols in the range from <1 to 100 mSv.

Compliance with ethical standards. The study was conducted in accordance with the Helsinki Declaration of the World Medical Association “Ethical Principles of Conducting Medical Research with human participation as a subject”, adopted at the 18th General Assembly of the WMA, Helsinki, Finland, June 1964 with all subsequent amendments and additions.

Contribution:
Okhrimenko S.E., Ingel F.I. — concept and design of the study, collection and processing of the material, writing text, editing;
Akhaltseva L.V., Yurtseva N.A., Krivtsova E.K., Nikitina T.A., Konyashkina M.A. — collection and processing of the material;
Ryzhkin S.A. — the concept and design of the study, editing;
Semenova M.P. — editing;
Blokhin I.A. — writing text;
Gombolevsky V.A. — concept and design of the study, collection and processing of the material, writing text.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgment. This work was prepared with the participation of the team as part of the medical research project (No. USIS [in the Unified State Information System for Accounting of Research, Development, and Technological Works]: 123031500005-2) «Scientific rationale for diagnostic radiology in oncology using radiomics».

Received: August 8, 2023 / Accepted: September 26, 2023 / Published: November 20, 2023

About the authors

Sergey E. Okhrimenko

Institution of Further Professional Education “Russian Medical Academy of Continuous Professional Education” of the Ministry of Healthcare of the Russian Federation;
State Research Center — Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency

Author for correspondence.
Email: ooniii@mail.ru
ORCID iD: 0000-0002-8282-1798

MD, Ph.D, Ass. Prof, Doctoral Student of the State Research Center of the Russian Federation, Burnasyan Federal Medical Biophysical Center, FMBA of Russia, Moscow, Russian Federation.

e-mail: ooniii@mail.ru

Russian Federation

Faina I. Ingel

Centre for Strategic Planning and Management of Biomedical Health Risks of the FMBA

Email: FIngel@cspmz.ru
ORCID iD: 0000-0002-2262-6800

Д.б.н., ведущий научный сотрудник отдела профилактической токсикологии и медико-биологических исследований Федерального государственного бюджетного учреждения «Центр стратегического планирования и управления медико-биологическими рисками здоровью» Федерального медико-биологического агентства, 119992, Москва Россия,

e-mail: FIngel@cspmz.ru

Russian Federation

Sergey A. Ryzhkin

Institution of Further Professional Education “Russian Medical Academy of Continuous Professional Education” of the Ministry of Healthcare of the Russian Federation;
Kazan State Medical University of the Ministry of Health of the Russian Federation

Email: rsa777@inbox.ru
ORCID iD: 0000-0003-2595-353X

Д.м.н., доцент, член-корреспондент АН Республики Татарстан, заведующий кафедрой радиотерапии и радиологии имени академика А.С. Павлова, профессор кафедры радиационной гигиены имени академика Ф.Г. Кроткова ФГБОУ ДПО РМАНПО Минздрава России,125284, Россия, город Москва, 2-й Боткинский проезд, дом 7; профессор кафедры общей гигиены ФГБОУ ВО Казанский ГМУ Минздрава России, 420012, Россия, город Казань, ул. Бутлерова, дом 49

e-mail: rsa777@inbox.ru

Russian Federation

Lyudmila V. Akhaltseva

Centre for Strategic Planning and Management of Biomedical Health Risks of the FMBA

Email: LAhalceva@cspmz.ru
ORCID iD: 0000-0002-3619-3858

Канд. биол. наук, старший научный сотрудник, отдел профилактической токсикологии и медико-биологических исследований. Федеральное государственное бюджетное учреждение «Центр стратегического планирования и управления медико-биологическими рисками здоровью» Федерального медико-биологического агентства России, Москва, 119121, Москва России

e-mail: LAhalceva@cspmz.ru

Russian Federation

Nadezhda A. Yurtseva

Centre for Strategic Planning and Management of Biomedical Health Risks of the FMBA

Email: NYUurceva@cspmz.ru
ORCID iD: 0000-0001-5031-2916

Младший научный сотрудник, отдел профилактической токсикологии и медико-биологических исследований. Федеральное государственное бюджетное учреждение «Центр стратегического планирования и управления медико-биологическими рисками здоровью» Федерального медико-биологического агентства России, 119121, Москва России

e-mail: NYUurceva@cspmz.ru

Russian Federation

Elena K. Krivtsova

Centre for Strategic Planning and Management of Biomedical Health Risks of the FMBA

Email: e_k_krivcova@mail.ru
ORCID iD: 0000-0002-5039-8980

Научный сотрудник, отдел профилактической токсикологии и медико-биологических исследований. Федеральное государственное бюджетное учреждение «Центр стратегического планирования и управления медико-биологическими рисками здоровью» Федерального медико-биологического агентства России, 119121, Москва

e-mail: e_k_krivcova@mail.ru

Russian Federation

Tatyana A. Nikitina

Centre for Strategic Planning and Management of Biomedical Health Risks of the FMBA

Email: TNikitina@cspmz.ru
ORCID iD: 0000-0003-0866-5990

Биолог, отдел профилактической токсикологии и медико-биологических исследований Федеральное государственное бюджетное учреждение «Центр стратегического планирования и управления медико-биологическими рисками здоровью» Федерального медико-биологического агентства России, 119121, Москва

e-mail: TNikitina@cspmz.ru

Russian Federation

Maria A. Konyashkina

Centre for Strategic Planning and Management of Biomedical Health Risks of the FMBA

Email: MKonyashkina@cspmz.ru
ORCID iD: 0000-0002-8319-1329

Канд. биол. наук, научный сотрудник, отдел профилактической токсикологии и медико-биологических исследований Федеральное государственное бюджетное учреждение «Центр стратегического планирования и управления медико-биологическими рисками здоровью» Федерального медико-биологического агентства России, 119121, Москва

e-mail: MKonyashkina@cspmz.ru

Russian Federation

Marija P. Semenova

Centre for Strategic Planning and Management of Biomedical Health Risks of the FMBA

Email: mps-fmbc@yandex.ru
ORCID iD: 0000-0003-0904-0415

Старший научный сотрудник лаборатории радиационной коммунальной гигиены Федерального государственного бюджетного учреждения «Государственный научный центр Российской Федерации — Федеральный медицинский биофизический центр имени А.И. Бурназяна», 123098, Москва, ул. Живописная, д. 46

e-mail: mps-fmbc@yandex.ru

Russian Federation

Ivan A. Blokhin

Moscow Center for Diagnostics and Telemedicine

Email: fake@neicon.ru
ORCID iD: 0000-0002-2681-9378
Russian Federation

Victor А. Gombolevskiy

Moscow Center for Diagnostics and Telemedicine;
Artificial Intelligence Research Institute (AIRI)

Email: gombolevskiy@airi.net
ORCID iD: 0000-0003-1816-1315
SPIN-code: 6810-3279

Кандидат медицинских наук, ведущий научный сотрудник АНО «Институт искусственного интеллекта» (AIRI), 121165, Москва, Россия

e-mail: gombolevskiy@airi.net

eLibrary SPIN: 6810-3279

Russian Federation

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