Partenogenesis Maize genes: Comparative Mutations Analysis

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Abstract

The article presents an analysis of the polymorphism of nucleotide sequences of the genes presumably associated with the parthenogenetic development of the embryo and endosperm in maize. Sequencing and subsequent multiple alignment of transcripts of the target genes (Hdt104, Chr106, Fie1 and Fie2) studied in the work of the AT-1, AT-3 and AT-4 parthenogenetic maize lines and the reference line B73 determined the presence of SNP, deletions and insertions. The phylogenetic trees for the studied genes were constructed.

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About the authors

E. M. Moiseeva

Institute of Biochemistry and Physiology of Plants and Microorganisms, Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”

Email: chumakov_m@ibppm.ru
Russian Federation, Saratov, 410049

V. V. Fadeev

Institute of Biochemistry and Physiology of Plants and Microorganisms, Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”

Email: chumakov_m@ibppm.ru
Russian Federation, Saratov, 410049

Yu. V. Fadeeva

Institute of Biochemistry and Physiology of Plants and Microorganisms, Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”

Email: chumakov_m@ibppm.ru
Russian Federation, Saratov, 410049

S. I. Mazilov

Institute of Biochemistry and Physiology of Plants and Microorganisms, Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”

Email: chumakov_m@ibppm.ru
Russian Federation, Saratov, 410049

A. Y. Kolesova

Federal Center of Agriculture Research of the South-East Region

Email: chumakov_m@ibppm.ru
Russian Federation, Saratov, 410010

M. I. Chumakov

Institute of Biochemistry and Physiology of Plants and Microorganisms, Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”

Author for correspondence.
Email: chumakov_m@ibppm.ru
Russian Federation, Saratov, 410049

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2. Fig. 1. The embryo sac (ES) of diploid maize line AT-4, containing the parthenogenetic embryo and endosperm. Unpollinated ES was isolated 10 days after isolation of the pistil threads. Field season 2022. The parthenogenetic (36-cell) embryo is shown by the thin arrow; the 4-nucleus parthenogenetic endosperm is shown by the thick arrow. Magnification × 400, microscope – Axio Scope A1 (Karl Zeiss, Germany).

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3. Fig. 2. Alignment of nucleotide sequences of Zm_Chr106 gene transcripts of KM, AT-3, and AT-4 lines and the reference line B73. Dots indicate nucleotide similarity to the sequence of the reference line B73; ONZ in KM, ZMS-8, and ZMS-P lines are designated by letters; a hyphen indicates the absence of a nucleotide, a frame indicates a nucleotide insertion; the figure shows gene fragments in which mutations were detected.

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4. Fig. 3. Multiple alignment of nucleotide sequences of the Zm_Fie1 gene of the Saratov-bred maize lines and the reference line B73. Dots indicate nucleotide similarity to the sequence of the reference line B73; ONZ in the lines AT-1, AT-3, AT-4 are designated by letters; a hyphen indicates the absence of a nucleotide; the figure shows gene fragments in which mutations were detected.

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5. Fig. 4. Multiple alignment of the fragment of nucleotide sequences of the Zm_Fie2 gene of parthenogenetic maize lines AT of Saratov selection and the reference line B73. The highlighted letters indicate nucleotide substitutions.

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6. Fig. 5. Phylogram of maize lines constructed based on the Zm_Chr106 gene using the MEGA 11 program. The lines studied in this article are marked with squares.

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7. Fig. 6. Phylogram of maize lines constructed based on the Zm_Fie1 gene using the MEGA 11 program. The lines studied in this article are marked with squares.

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