Genetic Diversity of the Field Vole Microtus agrestis (Arvicolinae, Rodentia) in the Central Part of Northern Eurasia According to the Cytochrome b Gene Data

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Abstract

An analysis of the genetic diversity of the field vole was carried out with the inclusion of new data on the complete sequences of the cytochrome b gene from the least studied area of the species range – the central part of Northern Eurasia (20 localities of the East European Plain, the Urals, Western and Eastern Siberia). The obtained results are generally consistent with modern view on the species phylogeography, but indicate the previously not investigated genetic heterogeneity of the Eastern clade, which occupies the entire Asian part of the species’ range and its European part to Northern and Eastern Europe. The comparison of the genetic diversity analysis results with the age of paleontological findings, carried out using the Urals territory as the example, allows to assume that climatic changes in the late Pleistocene and Holocene could not lead to the complete extinction of the species in the region, but to the spatial redistribution of its populations and to the complete or partial replacement of some genetic groups by others.

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About the authors

L. E. Yalkovskaya

Institute of Plant and Animal Ecology, Ural Branch of the Russian Academy of Sciences

Author for correspondence.
Email: lida@ipae.uran.ru
Russian Federation, Ekaterinburg

P. A. Sibiryakov

Institute of Zoology of the Republic of Kazakhstan

Email: lida@ipae.uran.ru
Kazakhstan, Almaty

M. A. Krohaleva

Institute of Plant and Animal Ecology, Ural Branch of the Russian Academy of Sciences

Email: lida@ipae.uran.ru
Russian Federation, Ekaterinburg

E. A. Markova

Institute of Plant and Animal Ecology, Ural Branch of the Russian Academy of Sciences

Email: lida@ipae.uran.ru
Russian Federation, Ekaterinburg

A. V. Borodin

Institute of Plant and Animal Ecology, Ural Branch of the Russian Academy of Sciences

Email: lida@ipae.uran.ru
Russian Federation, Ekaterinburg

S. A. Borisov

Irkutsk Antiplague Research Institute of Siberia and Far East

Email: lida@ipae.uran.ru
Russian Federation, Irkutsk

M. V. Chibiryak

Institute of Plant and Animal Ecology, Ural Branch of the Russian Academy of Sciences

Email: lida@ipae.uran.ru
Russian Federation, Ekaterinburg

A. V. Bobretsov

Pechora-Ilych State Nature Reserve

Email: lida@ipae.uran.ru
Russian Federation, Yaksha

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Supplementary files

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2. Fig. 1. M. agrestis areal [3], points of collection of new material and geographical localisation of the GenBank database data analysed in this work

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3. Fig. 2. Phylogenetic tree of M. agrestis reconstructed by Bayesian analysis for 348 cytb haplotypes (1140 bp). I-VI - clades: I - Eastern, II - Central European, III - Scandinavian, IV - Western European, V - French, VI - North British. Ia-Ic - groups of haplotypes of the Eastern clade. Black circles - original haplotypes (Magr1-Magr37). Numbers above branches are posterior probabilities (BI > 0.70). Circles with numbers on the branches of haplogroup Ic - posterior probabilities (BI > 0.65) for the subgroups including haplotypes from the Ural territory

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4. Fig. 3. Median network of 348 cytb haplotypes (1140 bp) of M. agrestis. I-VI clades: I - Eastern, II - Central European, III - Scandinavian, IV - Western European, V - French, VI - North British. Ia-Ic - haplogroups of the Eastern clade. Black circles - original haplotypes (Magr1-Magr37)

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5. Fig. 4. Geographical position of haplogroups of the Eastern clade of M. agrestis

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6. Fig. 5. Haplotype groups detected in M. agrestis in three localities of the Northern, five localities of the Middle and five localities of the Southern Urals (a) and the diversity of landscapes (b) in which the species occurs (+) or does not occur (-) in the Northern, Middle and Southern Urals (on the example of the western slope)

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