Genetic Variability of the Laxmann’s Shrew (Sorex caecutiens Laxmann, 1788) of the Kolyma River Basin and Chukotka

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Abstract

Nucleotide sequence polymorphism and haplotypic diversity of the cytochrome b gene were studied in samples of the Laxmann’s Shrew Sorex caecutiens koreni from the populations of the Kolyma River basin and Chukotka. 56 cytb-haplotypes differing among themselves by 87 mutations in 84 sites were found. The cytb-haplotypes of individuals of the subspecies S. c. koreni and S. c. macropygmaeus were shown to belong to the same maternal mtDNA lineage, their monophyletic origin from the same ancestral haplotype Scb1, and the presence of identical cytb-haplotypes in both subspecies. The mutual isolation of the shrew populations in the upper basin of the Kolyma River and Chukotka is shown. The proportion of intrapopulation genetic variability is 95.74%, intergroup variability is 5.74%, and intragroup variability is –1.48%, which reflects a very high level of intrapopulation variability of cytb haplotypes in the studied groups of S. c. koreni, their monophyletic origin, and the genetic unity of the Chukchi samples. The values of molecular diversity indices testify to the stability of populations that have a high value of effective abundance for a long time.

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V. V. Pereverzeva

Institute of Biological Problems of the North, Far Eastern Branch of the Russian Academy of Sciences

Author for correspondence.
Email: vvpereverzeva@mail.ru
Russian Federation, Magadan

N. E. Dokuchaev

Institute of Biological Problems of the North, Far Eastern Branch of the Russian Academy of Sciences

Email: vvpereverzeva@mail.ru
Russian Federation, Magadan

A. A. Primak

Institute of Biological Problems of the North, Far Eastern Branch of the Russian Academy of Sciences

Email: vvpereverzeva@mail.ru
Russian Federation, Magadan

E. A. Dubinin

Institute of Biological Problems of the North, Far Eastern Branch of the Russian Academy of Sciences

Email: vvpereverzeva@mail.ru
Russian Federation, Magadan

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2. Fig. 1. Map-scheme of sampling sites of the Middle Storm-Petrel subspecies S. c. koreni. Numbers indicate: river basins 1 - Kulu, 2 - Detrin, 3 - Srednekan; 4 - vicinity of Urultun, Malyk and Momontai lakes; 5 - basin of Omolon river; 6 - vicinity of Anadyr

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3. Fig. 2. Haplotypes of the cytochrome b gene of the medium rodent S. c. koreni from the populations of the upper Kolyma and Chukotka. Nucleotide substitutions are presented relative to the sequence of the Scb1 variant. The substitution sites are shown from the beginning of the cytochrome b gene

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4. Fig. 3. Fraction of haplotypes of the cytochrome b gene in samples of the medium-sized rodent S. c. koreni from the populations of the upper Kolyma and Chukotka rivers

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5. Fig. 4. Median network of haplotypes of the cytochrome b gene of the medium rodent S. c. koreni from the populations of the upper Kolyma and Chukotka. The size of circles is proportional to the share of mtDNA variant. * - transit in the 1st codon position, ** - transit in the 2nd codon position, # - transversion in the 1st codon position, ### - transversion in the 3rd codon position. Numbers denote mutation sites from the beginning of cytochrome b gene; mv - median vector

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