Genetic Identification of Putative Hybrids Between Grey Wolf and Golden Jackal

Capa

Citar

Texto integral

Acesso aberto Acesso aberto
Acesso é fechado Acesso está concedido
Acesso é fechado Somente assinantes

Resumo

We describe the results of genetic analysis of 11 phenotypically deviant individuals of grey wolf (Canis lupus Linnaeus, 1758) sensu lato collected in Voronezh State Nature Biosphere Reserve (Chernozem zone of European Russia) and Dagestan (Northern Caucasus, Russia) putatively identified morphologically as hybrids between grey wolf and golden jackal (Canis aureus Linnaeus, 1758). By means of maternally inherited mtDNA (sequences of cytochrome b gene fragment) and paternal lineage markers ZfY no F1 wolf-jackal hybrids were identified. As well, possibility of classification of the studied individuals to next generation hybrids from crosses between different wolf-jackal F1s. However, attribution of these animals to complex hybrids such as various backcrosses cannot be rejected. From the results of analysis by a set of autosomal microsatellite loci we putatively diagnosed a single F2 hybrid. As well, we obtained data that can be considered as traces of hybridization between wolf and jackal in southern regions of European Russa, albeit direct indications of introgression between these species not found. At the same time, the results of both genetic and craniological studies could be interpreted as indication to hybridization between wolves and domestic dogs on the same territories.

Texto integral

Acesso é fechado

Sobre autores

P. Kazimirov

Vavilov Institute of General Genetics Russian Academy of Sciences; All-Russian Research Institute for Environmental Protection

Autor responsável pela correspondência
Email: farenklaw@gmail.com
Rússia, Moscow, 119991; Moscow, 117628

Yu. Belokon

Vavilov Institute of General Genetics Russian Academy of Sciences

Email: farenklaw@gmail.com
Rússia, Moscow, 119991

M. Belokon

Vavilov Institute of General Genetics Russian Academy of Sciences

Email: farenklaw@gmail.com
Rússia, Moscow, 119991

A. Mishin

Peskov Voronezhsky State Nature Biosphere Reserve

Email: farenklaw@gmail.com
Rússia, Voronoezh, 394080

V. Stakheev

Institute of Arid Zones, South Scientific Centre Russian Academy of Sciences

Email: farenklaw@gmail.com
Rússia, Rostov-on-Don, 344006

Yu. Yarovenko

Caspian Institute of Biological Resources, Dagestan Federal Research Centre Russian Academy of Sciences

Email: farenklaw@gmail.com
Rússia, Makhachkala, 367000

A. Yarovenko

Caspian Institute of Biological Resources, Dagestan Federal Research Centre Russian Academy of Sciences

Email: farenklaw@gmail.com
Rússia, Makhachkala, 367000

D. Politov

Vavilov Institute of General Genetics Russian Academy of Sciences; All-Russian Research Institute for Environmental Protection

Email: dmitri_p@inbox.ru
Rússia, Moscow, 119991; Moscow, 117628

Bibliografia

  1. Lindblad-Toh K., Wade C.M., Mikkelsen T.S. et al. Genome sequence, comparative analysis and haplotype structure of the domestic dog // Nature. 2005. V. 438. № 7069. P. 803–819. https://doi.org/10.1038/nature04338
  2. Galov A., Fabbri E., Caniglia R. et al. First evidence of hybridization between golden jackal (Canis aureus) and domestic dog (Canis familiaris) as revealed by genetic markers // Royal Society Open Science. 2015. V. 2. № 12. https://doi.org/10.1098/rsos.150450
  3. Rykov A.M., Kuznetsova A.S., Tirronen K.F. The first record of the golden jackal (Canis aureus Linnaeus, 1758) in the Russian Subarctic // Polar Biology. 2022. V. 45. № 5. P. 965–970.
  4. Stronen A.V., Bartol M., Boljte B. et al. “Passive surveillance” across species with cross-amplifying molecular markers: the potential of wolf (Canis lupus) genetic monitoring in tracking golden jackal (C. aureus) colonization and hybridization // Hystrix, the Italian J. Mammalogy. 2020. V. 31. № 1. P. 74–76. http://dx.doi.org/10.4404/hystrix-00259-2019
  5. Trouwborst A., Krofel M., Linnell J.D.C. Legal implications of range expansions in a terrestrial carnivore: the case of the golden jackal (Canis aureus) in Europe // Biodiversity and Conservation. 2015. V. 24. № 10. P. 2593–2610. https://doi.org/10.1007/s10531-015-0948-y
  6. Новиков Г.А. Хищные млекопитающие фауны СССР. М.; Л.: АН СССР, 1956. 294 с.
  7. Волк. Происхождение, систематика, морфология, экология / Под ред. Бибикова Д.И. М.: Наука, 1985. 606 c.
  8. Кудактин А.Н., Яровенко Ю.А., Яровенко А.Ю. Современное распространение и экология шакала обыкновенного Canis aureus (L., 1758) в России и на Кавказе // Вестник охотоведенья. 2019. Т. 16. № 1, С. 22–-28.
  9. Rutledge L.Y., White B.N., Row J.R., Patterson B.R. Intense harvesting of eastern wolves facilitated hybridization with coyotes // Ecol. Evol. 2012. V. 2. № 1. P. 19–33. https://doi.org/10.1002%2Fece3.61
  10. Bohling J.H., Waits L. P. Factors influencing red wolf–coyote hybridization in eastern North Carolina, USA // Biol. Conservation. 2015. V. 184. P. 108–116. https://doi.org/10.1016/j.biocon.2015.01.013
  11. Leonard J.A., Echegaray J., Randi E., Vila C. Impact of hybridization with domestic dogs on the conservation of wild canids // Free-Ranging Dogs and Wildlife Conservation / Ed. Gompper M.E., Oxford: Oxford Univ. Press, 2014. P. 170-184.
  12. Barash A., Preiss-Bloom S., Machluf Y. et al. Possible origins and implications of atypical morphologies and domestication-like traits in wild golden jackals (Canis aureus) // Sci. Reports. 2023. V. 13:7388. https://doi.org/10.1038/s41598-023-34533-w
  13. Хейдорова Е.Э., Шпак А.В., Гомель К.В. и др. Молекулярно-генетическая идентификация инвазивного вида – шакала азиатского (Canis aureus) на территории Беларуси // Докл. Нац. академии наук Беларуси. 2018. Т. 62. № 1. С. 86–92.
  14. Рябов Л.С. Волки Черноземья. Воронеж: Изд-во ВГУ, 1993. 168 с.
  15. Smith M.F., Patton J.L. Variation in mitochondrial cytochrome b sequence in natural populations of South American akodontine rodents (Muridae: Sigmodontinae) // Mol. Biol. Evol. 1991. V. 8. № 1. P. 85-103. https://doi.org/10.1093/oxfordjournals.molbev.a040638
  16. Boonyaprakob U., Homsavart S., Noosud J., Tungtrakanpoung R. Cloning and comparative analysis of zinc-finger protein gene on Y-chromosome (ZFY) between Thai Bangkaew dog and other Thai canids // Agriculture and Nat. Resources. 2017. V. 51. № 3. P. 212–217. http://dx.doi.org/10.1016/j.anres.2016.12.007
  17. Galov A., Sindicic M., Gomercic T. et al. PCR-based Y chromosome marker for discriminating between golden jackal (Canis aureus) and domestic dog (Canis lupus familiaris) paternal ancestry // Conservation Genet. Resources. 2014. V. 6. № 2. P. 275–277. https://doi.org/10.1007/s12686-013-0110-7
  18. Akesson M., Liberg O., Sand H. et al. Genetic rescue in a severely inbred wolf population // Mol. Ecol. 2016. V. 25. № 19. P. 4745–4756. https://doi.org/10.1111/mec.13797
  19. Казимиров П.А., Леонтьев С.В., Нечаева А.В. и др. Популяционно-генетическая структура степного волка России и Казахстана по микросателлитным локусам // Генетика. 2022. Т. 58. № 11. С. 1261–1272. https://doi.org/10.31857/S0016675822110042
  20. Anderson E., Thompson E. A model-based method for identifying species hybrids using multilocus genetic data // Genetics. 2002. V. 160. № 3. P. 1217–1229. https://doi.org/10.1093/genetics/160.3.1217
  21. Adams D.C., Collyer M.L., Kaliontzopoulou A., Baken E.K. Geomorph: Software for Geometric Morphometric Analyses. R package version 4.0.6. 2023. https://cran.r-project.org/package=geomorph
  22. Collyer M.L., Adams D.C. {RRPP}: Linear model evaluation with randomized residuals in a permutation procedure // Meth. Ecol. Evol. 2023. V. 9. № 2. P. 1772–1779. https://doi.org/10.1111/2041-210X.13029
  23. R Core Team. R: A Language and Environment for Statistical Computing // 2022. https://www.R-project.org
  24. Harmoinen J., von Thaden A., Aspi J. et al. Reliable wolf-dog hybrid detection in Europe using a reduced SNP panel developed for non-invasively collected samples // BMC Genomics. 2021. V. 22:473. https://doi.org/10.1186/s12864-021-07761-5

Arquivos suplementares

Arquivos suplementares
Ação
1. JATS XML
2. Fig. 1. The result of electrophoresis of PCR products obtained using primer kits dZFY1F/dZFY2R and YintF2/YintR/Yint2-335. C. a. – Canis aureus, C. l. – Canis lupus, M – dimensional standard 1 kb, fragment lengths in mon.

Baixar (132KB)
3. Fig. 2. Results of craniometric analysis of 3D models using the principal component method.

Baixar (74KB)

Declaração de direitos autorais © Russian Academy of Sciences, 2024