Genetic characteristics of the Gray Mountain Caucasian Bee Apis mellifera caucasica
- Authors: Kaskinova M.D.1, Gaifullina L.R.1, Saltykova E.S.1
-
Affiliations:
- Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Center of the Russian Academy of Sciences
- Issue: Vol 60, No 8 (2024)
- Pages: 122-126
- Section: КРАТКИЕ СООБЩЕНИЯ
- URL: https://rjpbr.com/0016-6758/article/view/667225
- DOI: https://doi.org/10.31857/S0016675824080125
- EDN: https://elibrary.ru/bfgghw
- ID: 667225
Cite item
Abstract
In this study we present the results of a comparative genetic analysis of bees of the Apis mellifera caucasica subspecies with the subspecies A. m. carnica and A. m. mellifera. We performed polymorphism analysis of nine microsatellite loci (Ap243, 4a110, A24, A8, A113, A88, Ap049, A28, and A43) and determined the haplotypes of the tRNAleu-COII locus. Analysis of the genetic structure of representatives of three subspecies of honey bees, widespread in Russia, showed a significant level of their differentiation even when using a small set of microsatellite loci. An assessment of the prevalence of tRNAleu-COII haplotypes in the three studied samples showed that for A. m. caucasica the predominant haplotype was C2j.
Full Text

About the authors
M. D. Kaskinova
Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Center of the Russian Academy of Sciences
Author for correspondence.
Email: kaskinovamilyausha@mail.ru
Russian Federation, Ufa, 450054
L. R. Gaifullina
Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Center of the Russian Academy of Sciences
Email: kaskinovamilyausha@mail.ru
Russian Federation, Ufa, 450054
E. S. Saltykova
Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Center of the Russian Academy of Sciences
Email: kaskinovamilyausha@mail.ru
Russian Federation, Ufa, 450054
References
- Горбачев К.А. Кавказская серая горная пчела (Apis mellifera var. caucasica) и место ее среди других пчел. Тифлис: тип. Труд, 1916. 39 с.
- Алпатов В.В. Породы медоносной пчелы. Москва: Изд-во моск. об-ва испытателей природы, 1948. 183 с.
- Ruttner F. Biogeography and Taxonomy of Honeybees. Berlin: Springer, 1988. 291 p.
- Любимов Е.М., Сокольский С.С., Савушкина Л.Н., Бородачев А.В. Селекция пчел серой горной кавказской породы и производство продукции в пчелоразведенческом хозяйстве. Рязань: Изд-во Ряз. обл. тип., 2013. 192 с.
- Cridland J.M., Tsutsui N.D., Ramirez S.R. The complex demographic history and evolutionary origin of the western honey bee Apis mellifera // Genome Biol. Evol. 2017. V. 9. P. 457–472. https://doi.org/10.1093%2Fgbe%2Fevx009
- Momeni J., Parejo M., Nielsen R.O., Langa J., et al. Authoritative subspecies diagnosis tool for European honey bees based on ancestry informative SNPs // BMC Genomics. 2021. V. 22. № 101. https://doi.org/10.1186/s12864-021-07379-7
- Garnery L., Solignac M., Celebrano G., Cornuet J.-M. A simple test using restricted PCR-amplified mitochondrial DNA to study the genetic structure of Apis mellifera L. // Experientia. 1993. V. 49. P. 1016–1021. https://doi.org/10.1007/BF02125651
- Cornuet J.M., Garnery L. Mitochondrial DNA variability in honeybees and its phylogeographic implications // Apidologie. 1991. V. 22. P. 627–642.
- Susnik S., Kozmus P., Poklukar J., Meglic V. Molecular characterisation of indigenous Apis mellifera carnica in Slovenia // Apidologie. 2004. V. 35. P. 623–636. https://doi.org/10.1051/apido:2004061
- Alburaki M., Madella S., Lopez J., et al. Honey bee populations of the USA display restrictions in their mtDNA haplotype diversity // Frontiers in Genetics. 2023. V. 13. https://doi.org/10.3389/fgene.2022.1092121
- Earl D.A., vonHoldt B.M. STRUCTURE HARVESTER: A website and program for visualizing STRUCTURE output and implementing the Evanno method // Conservation Genetics Resources. 2012. V. 4(2). P. 359–361. https://doi.org/10.1007/s12686-011-9548-7
- Evanno G., Regnaut S., Goudet J. Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study // Mol. Ecol. 2005. V. 14(8). P. 2611–2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x
- Nei M. Molecular еvolutionary пenetics // DNA Рolymorphism Within and Between Populations. N. Y.: Columbia Univ. Press, 1987. P. 254–285.
- Nikolova S.R., Bienkowska M., Gerula D., Ivanova E.N. Microsatellite DNA polymorphism in selectively controlled Apis mellifera carnica and Apis mellifera caucasica populations from Poland // Arch. Biol. Sci. 2015. V. 67(3). P. 889–894. https://doi.org/10.2298/ABS141102048N
- Tozkar C.O. Genetic structure of honey bee (Apis mellifera Linnaeus, 1758) subspecies based on tRNAleu-COX2 and ND5 regions of mtDNA // Applied Ecol. and Environ. Res. 2020. V. 18(2). https://doi.org/10.15666/aeer/1802_22692284
- Franck P., Garnery L., Celebrano G. et al. Hybrid origins of honeybees from Italy (Apis mellifera ligustica) and Sicily (A. m. sicula) // Mol. Ecol. 2000. V. 9. P. 907–921. https://doi.org/10.1046/j.1365-294x.2000.00945.x
- Carpenter M.H., Harpur B.A. Genetic past, present, and future of the honey bee (Apis mellifera) in the United States of America // Apidologie. 2021. V. 52. P. 63–79. https://doi.org/10.1007/s13592-020-00836-4
- Kaskinova M.D., Gaifullina L.R., Saltykova E.S. Haplotypes of the tRNAleu-COII mtDNA region in Russian Apis mellifera populations // Animals. 2023. V. 13. https://doi.org/10.3390/ani13142394
- Marcelino J., Braese C., Christmon K. et al. The movement of western honey bees (Apis mellifera L.) among U.S. States and territories: History, benefits, risks, and mitigation strategies // Front. Ecol. Evol. 2022. V. 10. https://doi.org/10.3389/fevo.2022.850600
- Collet T., Ferreira K., Arias M. et al. Genetic structure of Africanized honeybee populations (Apis mellifera L.) from Brazil and Uruguay viewed through mitochondrial DNA COI–COII patterns // Heredity. 2006. V. 97. P. 329–335. https://doi.org/10.1038/sj.hdy.6800875
- Oleksa A., Kusza S., Tofilski A. Mitochondrial DNA suggests the introduction of honeybees of african ancestry to East-Central Europe // Insects. 2021. V. 12. https://doi.org/10.3390/insects12050410
- Chavez-Galarza J., Lopez-Montanez R., Jimenez A. et al. Mitochondrial DNA variation in Peruvian honey bee (Apis mellifera L.) populations using the tRNAleu-cox2 intergenic region // Insects. 2021. V. 12. https://doi.org/10.3390/ insects12070641
- Tanasković M., Erić P., Patenković A. et al. MtDNA analysis indicates human-induced temporal changes of Serbian honey bees diversity // Insects. 2021. V. 12. https://doi.org/10.3390/insects12090767
- Salehi S., Nazemi-Rafie J. Discrimination of Iranian honeybee populations (Apis mellifera meda) from commercial subspecies of Apis mellifera L. using morphometric and genetic methods // J. of Asia-Pacific Entomology. 2020. № 23. P. 591–598. https://doi.org/10.1016/j.aspen.2020.04.009
- Chavez-Galarza J., Garnery L., Henriques D. et al. Mitochondrial DNA variation of Apis mellifera iberiensis: Further insights from a large scale study using sequence data of the tRNAleu-cox2 intergenic region // Apidologie. 2017. V. 48. P. 533–544.
Supplementary files
