Sequencing and Annotation of the Chloroplast Genome of Triticum militinae – a “Natural Mutant” of Tetraploid Wheat Triticum timopheevii Zhuk.

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Triticum militinae Zhuk. et Migusch. – tetraploid wheat with the GAA genome, considered a natural naked mutant of T. timopheevii Zhuk. Previously, experiments were conducted for this wheat to study the karyotype and crossing characteristics. To clarify its origin and relationship with other representatives of the wheat family, analysis of the chloroplast genome of T. militinae, which has previously remained unstudied, is of great interest. The sucrose gradient method was used to isolate chloroplast DNA from leaves. Sequencing was performed using the FASTASeq 300 Sequencing Kit V1.0 100 M reads/flow cell on a Genolab M sequencer (GeneMind, China). For the first time, we sequenced and annotated the complete chloroplast genome of T. militinae with a size of 135898 bp. In the structure of the plastid genome there are a pair of inverted repeats with a size of 21552 bp each, the region of a small single copy (SSC) – 12791 bp and the region of a large single copy (LSC) – 80003 bp. As part of the chloroplast genome of T. militinae 132 structural genes were found, of which 85 protein-coding genes, 31 tRNAs and 4 rRNA genes.

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Sobre autores

A. Kuluev

Institute of Biochemistry and Genetics – Subdivision of the Federal State Budgetary Scientific Institution of the Ufa Federal Research Center of the Russian Academy of Sciences

Autor responsável pela correspondência
Email: kuluev.azat91@yandex.ru
Rússia, Ufa, 450054

R. Matniyazov

Institute of Biochemistry and Genetics – Subdivision of the Federal State Budgetary Scientific Institution of the Ufa Federal Research Center of the Russian Academy of Sciences

Email: kuluev.azat91@yandex.ru
Rússia, Ufa, 450054

B. Kuluev

Institute of Biochemistry and Genetics – Subdivision of the Federal State Budgetary Scientific Institution of the Ufa Federal Research Center of the Russian Academy of Sciences

Email: kuluev.azat91@yandex.ru
Rússia, Ufa, 450054

L. Privalov

Institute of Biochemistry and Genetics – Subdivision of the Federal State Budgetary Scientific Institution of the Ufa Federal Research Center of the Russian Academy of Sciences

Email: kuluev.azat91@yandex.ru
Rússia, Ufa, 450054

A. Chemeris

Institute of Biochemistry and Genetics – Subdivision of the Federal State Budgetary Scientific Institution of the Ufa Federal Research Center of the Russian Academy of Sciences

Email: kuluev.azat91@yandex.ru
Rússia, Ufa, 450054

Bibliografia

  1. Жуковский П.М., Мигушова Э.Ф. Наиболее высокоиммунный эндемичный генофонд для выведения устойчивых сортов пшеницы путем отдаленной гибридизации // Вестник с.-х. наук. 1969. № 2. С. 9–20.
  2. Кулуев А.Р., Матниязов Р.Т., Кулуев Б.Р., Чемерис А.В. Triticum militinae Zhuk. et Migusch. – точно не мутант T. timopheevii Zhuk., как считалось долгие годы // Biomics. 2023. V. 15. № 3. P. 213–217. https://doi.org/10.31301/2221-6197.bmcs.2023-19
  3. Наврузбеков Н.А. К происхождению Triticum militinae Zhuk. et Migusch. // Ботанические и генетические ресурсы флоры Дагестана. Махачкала: Даг. фил. АН СССР, 1981. С. 121–122.
  4. Shi C., Hu N., Huang H. et al. An improved chloroplast DNA extraction procedure for whole plastid genome sequencing // PLoS One. 2012. V. 7. № 2. https://doi.org/10.1371/journal.pone.0031468
  5. Graham D.E. The isolation of high molecular weight DNA from whole organisms of large tissue masses // Anal. Biochem. 1978. V. 78. P. 673–678.
  6. Bolger A.M., Lohse M., Usadel B. Trimmomatic: A flexible trimmer for Illumina sequence data // Bioinformatics. 2014. V. 30. P. 2114–2120. https://doi.org/10.1093/bioinformatics/btu170
  7. Wu P., Xu C., Chen H. et al. NOVOWrap: An automated solution for plastid genome assembly and structure standardization // Mol. Ecol. Res. 2021. V. 21(6). P. 2177–2186. https://doi.org/10.1111/1755-0998.13410
  8. Shi L., Chen H., Jiang M. et al. CPGAVAS2, an integrated plastome sequence annotator and analyzer // Nucl. Acids Res. 2019. V. 47. P. W65–W73. https://doi.org/10.1093/nar/gkz345
  9. Greiner S., Lehwark P., Bock R. Organellar Genome DRAW (OGDRAW) version 1.3.1: expanded toolkit for the graphical visualization of organellar genomes // Nucl. Acids. Res. 2019. V. 47. P. W59–W64.

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2. Fig. 1. Representation of the chloroplast genome of T. militinae var. albimilitinae k-59942 as a ring. Plastome visualization was performed using the OGDRAW resource. Genes are shown in different colors, the gray circle in the middle shows the level of GC sites. IRA is the inverted repeat region A, IRB is the inverted repeat region B. Genes located outside the outer circle are transcribed clockwise, and genes located inside are transcribed counterclockwise.

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