Sequencing and Annotation of the Chloroplast Genome of Triticum militinae – a “Natural Mutant” of Tetraploid Wheat Triticum timopheevii Zhuk.
- Autores: Kuluev A.R.1, Matniyazov R.T.1, Kuluev B.R.1, Privalov L.Y.1, Chemeris A.V.1
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Afiliações:
- Institute of Biochemistry and Genetics – Subdivision of the Federal State Budgetary Scientific Institution of the Ufa Federal Research Center of the Russian Academy of Sciences
- Edição: Volume 60, Nº 8 (2024)
- Páginas: 118-121
- Seção: КРАТКИЕ СООБЩЕНИЯ
- URL: https://rjpbr.com/0016-6758/article/view/667224
- DOI: https://doi.org/10.31857/S0016675824080114
- EDN: https://elibrary.ru/bfgxhu
- ID: 667224
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Resumo
Triticum militinae Zhuk. et Migusch. – tetraploid wheat with the GAA genome, considered a natural naked mutant of T. timopheevii Zhuk. Previously, experiments were conducted for this wheat to study the karyotype and crossing characteristics. To clarify its origin and relationship with other representatives of the wheat family, analysis of the chloroplast genome of T. militinae, which has previously remained unstudied, is of great interest. The sucrose gradient method was used to isolate chloroplast DNA from leaves. Sequencing was performed using the FASTASeq 300 Sequencing Kit V1.0 100 M reads/flow cell on a Genolab M sequencer (GeneMind, China). For the first time, we sequenced and annotated the complete chloroplast genome of T. militinae with a size of 135898 bp. In the structure of the plastid genome there are a pair of inverted repeats with a size of 21552 bp each, the region of a small single copy (SSC) – 12791 bp and the region of a large single copy (LSC) – 80003 bp. As part of the chloroplast genome of T. militinae 132 structural genes were found, of which 85 protein-coding genes, 31 tRNAs and 4 rRNA genes.
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Sobre autores
A. Kuluev
Institute of Biochemistry and Genetics – Subdivision of the Federal State Budgetary Scientific Institution of the Ufa Federal Research Center of the Russian Academy of Sciences
Autor responsável pela correspondência
Email: kuluev.azat91@yandex.ru
Rússia, Ufa, 450054
R. Matniyazov
Institute of Biochemistry and Genetics – Subdivision of the Federal State Budgetary Scientific Institution of the Ufa Federal Research Center of the Russian Academy of Sciences
Email: kuluev.azat91@yandex.ru
Rússia, Ufa, 450054
B. Kuluev
Institute of Biochemistry and Genetics – Subdivision of the Federal State Budgetary Scientific Institution of the Ufa Federal Research Center of the Russian Academy of Sciences
Email: kuluev.azat91@yandex.ru
Rússia, Ufa, 450054
L. Privalov
Institute of Biochemistry and Genetics – Subdivision of the Federal State Budgetary Scientific Institution of the Ufa Federal Research Center of the Russian Academy of Sciences
Email: kuluev.azat91@yandex.ru
Rússia, Ufa, 450054
A. Chemeris
Institute of Biochemistry and Genetics – Subdivision of the Federal State Budgetary Scientific Institution of the Ufa Federal Research Center of the Russian Academy of Sciences
Email: kuluev.azat91@yandex.ru
Rússia, Ufa, 450054
Bibliografia
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