Nematode Caenorhabditis elegans as an object for testing the genotoxicity of chemical compounds
- Authors: Abilev S.K.1, Machigov E.M.1, Smirnova S.V.1, Marsova M.V.1
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Affiliations:
- Vavilov Institute of General Genetics Russian Academy of Science
- Issue: Vol 60, No 9 (2024)
- Pages: 10-15
- Section: ОБЩАЯ ГЕНЕТИКА
- URL: https://rjpbr.com/0016-6758/article/view/667194
- DOI: https://doi.org/10.31857/S0016675824090027
- EDN: https://elibrary.ru/afavql
- ID: 667194
Cite item
Abstract
In connection with the requirements of International and national organizations to comply with the principles of humanization of experiments using animals, alternative test systems are being searched and tested to replace animals in ecological, toxicological and genotoxicological studies. One such alternative may be the nematode Caenorhabditis elegans, which has a biotransformation system of chemical compounds similar to the mammalian system. The genotoxicity of the pesticides paraquat and the antibacterial agent furacilin was studied on C. elegans by horizontal gel electrophoresis of total DNA in order to assess its integrity. It has been shown that paraquat in concentrations of 0.01 to 0.05 mol/l and furacilin f in concentrations of 0.0001 and 0.00025 mol/l, caused DNA breaks in nematode cells. The antioxidant N-acetylcysteine at a concentration of 0.01 mol/l reduced the genotoxicity of both compounds.
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About the authors
S. K. Abilev
Vavilov Institute of General Genetics Russian Academy of Science
Author for correspondence.
Email: abilev@vigg.ru
Russian Federation, Moscow, 119991
E. M. Machigov
Vavilov Institute of General Genetics Russian Academy of Science
Email: abilev@vigg.ru
Russian Federation, Moscow, 119991
S. V. Smirnova
Vavilov Institute of General Genetics Russian Academy of Science
Email: abilev@vigg.ru
Russian Federation, Moscow, 119991
M. V. Marsova
Vavilov Institute of General Genetics Russian Academy of Science
Email: abilev@vigg.ru
Russian Federation, Moscow, 119991
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