Expression of the LYST and SLFN12L Genes in Peripheral Blood Leukocytes in Patients with Chronic Pulmonary Sarcoidosis

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Abstract

The expression of the LYST and SLFN12L genes in peripheral blood leukocytes (PBL) in patients with pulmonary sarcoidosis with a chronic course of the disease and in healthy people was studied in this work. Patients with chronic pulmonary sarcoidosis, on the second stage of the disease (20 people without therapy, average age of whom was 41.00 ± 12.56 years) and 25 healthy people (average age was 45.86 ± 2.13 years) were enrolled in this study. The level of gene expression was determined by real-time PCR. A significant decrease in the transcripts of the SLFN12L gene (p = 0.002) and a low content of mRNA of the LYST gene (p = 0.09) in the PBL of patients with pulmonary sarcoidosis compared to healthy people was established. Differential expression of LYST and SLFN12L may indicate the involvement of these genes in the pathogenesis of this disease, and it is possible they participate in the modulation of immune reactions during the development of inflammatory processes in pulmonary sarcoidosis.

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About the authors

I. E. Malysheva

Karelian Research Centre of the Russian Academy of Sciences

Author for correspondence.
Email: i.e.malysheva@yandex.ru
Russian Federation, Petrozavodsk, 185910

O. V. Balan

Karelian Research Centre of the Russian Academy of Sciences

Email: i.e.malysheva@yandex.ru
Russian Federation, Petrozavodsk, 185910

E. L. Tikhonovich

Karelian Research Centre of the Russian Academy of Sciences; Baranov Republican Hospital

Email: i.e.malysheva@yandex.ru
Russian Federation, Petrozavodsk, 185910; Petrozavodsk, 185019

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2. Fig. 1. The expression level of the genes SLFN12L (a), LYST (b), LC3B2 (c), p53 (d) and ATG5 (e) in the PBL of conditionally healthy donors (1) and patients with pulmonary sarcoidosis (2). The horizontal lines inside the rectangles are the median, • is the mean value.

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