Targeted Sequencing of the LEP Gene in Various Ethnic Groups of Obese Adolescents

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Abstract

The gene encoding leptin (LEP) is otherwise called the obesity factor gene. It has been shown that polymorphic loci and mutations in the LEP gene can cause metabolic disorders and lead to obesity, as well as the development of various pathologies associated with obesity. The purpose of this study was to search for polymorphic variants of the LEP gene in groups of overweight and/or obese adolescents in ethnic samples of Russians and Buryats. The study involved 48 adolescents aged 11 to 17 years (average age 14.27 ± 2.09 years) with different weight status: normal body weight and overweight and/or obesity. Of these, 21 were Russians and 27 were Buryats. The research methods included: assessment of the clinical status and anthropometric indicators; polymerase chain reaction (PCR) and sequencing using the Sanger method of a fragment of the LEP gene; bioinformatic analysis; statistical processing of the results obtained. In the study, amplification conditions were selected for a fragment of the LEP gene with a total length of 3878 bp. (128251456–128255334) and its sequencing was carried out. After bioinformatic processing of the obtained results, ten SNPs were found in the studied group of adolescents with different weight status, seven of them registered in the NCBI database and three replacements not previously registered in the NCBI. Of the seven polymorphisms registered in the NCBI, one SNP was identified in all study groups, three SNPs were identified in the main group, one of them in the Russian group, one in the Buryat group and one in both ethnic groups, and three SNPs were found only in the control group in Buryat adolescents. Three SNPs not registered with the NCBI were identified only in the overweight and/or obese adolescents group, including two in the Russian group chr7:128255051 (G > C), chr7:128255092 (G > C) and one in the Buryat group chr7:128254681 (C > G). The study characterized the frequency of SNPs identified as a result of sequencing of a fragment of the LEP gene in groups of adolescents with different weight status.

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E. V. Belyaeva

Scientific Сentre for Family Health and Human Reproduction Problems

Author for correspondence.
Email: belyeva_irk@mail.ru
Russian Federation, Irkutsk, 664003

T. A. Bairova

Scientific Сentre for Family Health and Human Reproduction Problems

Email: belyeva_irk@mail.ru
Russian Federation, Irkutsk, 664003

O. A. Ershova

Scientific Сentre for Family Health and Human Reproduction Problems

Email: belyeva_irk@mail.ru
Russian Federation, Irkutsk, 664003

A. Yu. Sambyalova

Scientific Сentre for Family Health and Human Reproduction Problems

Email: belyeva_irk@mail.ru
Russian Federation, Irkutsk, 664003

V. V. Sinkov

Scientific Сentre for Family Health and Human Reproduction Problems

Email: belyeva_irk@mail.ru
Russian Federation, Irkutsk, 664003

V. V. Balzhieva

Scientific Сentre for Family Health and Human Reproduction Problems

Email: belyeva_irk@mail.ru
Russian Federation, Irkutsk, 664003

L. V. Rychkova

Scientific Сentre for Family Health and Human Reproduction Problems

Email: belyeva_irk@mail.ru
Russian Federation, Irkutsk, 664003

L. I. Kolesnikova

Scientific Сentre for Family Health and Human Reproduction Problems

Email: belyeva_irk@mail.ru
Russian Federation, Irkutsk, 664003

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