Molecular genetic аnd meiotic peculiarities оf Caucasus pine vole Microtus (Terricola) daghestanicus in the eastern part of the Greater Caucasus

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Abstract

The high level of karyotypic and molecular genetic variability was previously detected in the Caucasus pine vole Microtus daghestanicus. However, populations of this species were poorly studied in the eastern part of the Greater Caucasus. To reveal their genetic peculiarities and phylogenetic relationships with other conspecific populations we analyzed a sample of Caucasus pine voles from the south of the Chechen Republic (Daikhokh Mount). The immunocytochemical study of synaptonemal complexes in meiotic prophase I demonstrated that the population belongs to the most distributed 54-chromosomal form of M. daghestanicus. The entire mitochondrial cytochrome b gene analysis showed close clustering specimens from Chechen Republic and previously studied Caucasus pine vole from northern Georgia. It indicates that all these specimens belong to a discrete genetic form. In the studied sample from Chechen Republic we revealed variability of the nuclear BRCA1 and XIST genes that probably reflects a complicated history of the population associated with alternation of its isolation from neighbor populations and restoring contacts between them.

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About the authors

A. S. Bogdanov

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: sergey8585@mail.ru
Russian Federation, Moscow, 119334

M. M. Atsaeva

Ibragimov Complex Institute of the Russian Academy of Sciences; Vavilov Institute of General Genetics, Russian Academy of Sciences

Email: sergey8585@mail.ru
Russian Federation, Grozny, 344051; Moscow, 119991

D. D. Arsanukaev

Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences

Email: sergey8585@mail.ru
Russian Federation, Moscow, 119071

S. N. Matveevsky

Vavilov Institute of General Genetics, Russian Academy of Sciences

Author for correspondence.
Email: sergey8585@mail.ru
Russian Federation, Moscow, 119991

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Supplementary files

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2. Fig. 1. The sprawled nucleus of the spermatocyte of the Dagestan vole M. daghestanicus (No. T23-01), the pachytene stage. Immunodetection of the protein of the lateral elements of SC – SYCP3 (a), kinetochore protein (centromeres) was performed using CREST antibodies (b). Chromatin was stained with DAPI (c). The combined image of the SYCP3 and centromere proteins is presented separately (d). Some areas of chromatin between the SC are CREST-positive (marked with arrows). The scale is 5 microns.

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3. Fig. 2. ML-dendrograms constructed when comparing Dagestan voles from different populations and several individuals of M. subterraneus and M. majori according to the complete sequence of the cytb mitochondrial gene (a), the combined sequence of two non-overlapping fragments of the XIST nuclear gene (b) and a fragment of the 11th exon of the BRCA1 nuclear gene (c). In the nodes tree branches are given bootstrap index values exceeding 70%. For previously published sequences, GenBank database numbers are indicated; numbers without additional designations are taken from the publication [7], numbers marked with an asterisk are from the article [9]. For the XIST gene, the numbers corresponding to its first and second separately sequenced fragments are separated by commas.

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