Assessing the efficacy of anti-cancer drugs on organoid models derived from prostate cancer

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It has been proven that tumor organoids effectively mirror the phenotypic and genetic traits of the original biomaterial. It has been noted that outcomes from drug testing in organoid cultures can accurately represent the clinical response observed in patients. In this study, an organoid culture was derived from biopsy material of prostate cancer (PC). Subsequently, clinical practice drugs, docetaxel and enzalutamide, were tested on this organoid culture. Various techniques for evaluating the efficacy of drugs in vitro were compared. The half maximal inhibitory concentration of docetaxel was found to be markedly lower compared to that of enzalutamide. However, when tested at clinically relevant concentrations and incubation times, enzalutamide was more effective than docetaxel. Therefore, it is crucial to optimize the testing conditions for drugs on in vitro cultures for their subsequent application in clinical practice.

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作者简介

M. Silkina

National Research University “Higher School of Economics”

编辑信件的主要联系方式.
Email: mosilkina@hse.ru
俄罗斯联邦, Moscow

A. Razumovskaya

National Research University “Higher School of Economics”

Email: mosilkina@hse.ru
俄罗斯联邦, Moscow

S. Nikulin

National Research University “Higher School of Economics”; National Medical Research Radiological Center, Ministry of Health of Russia

Email: mosilkina@hse.ru
俄罗斯联邦, Moscow; Moscow

A. Tonevitsky

National Research University “Higher School of Economics”

Email: mosilkina@hse.ru
俄罗斯联邦, Moscow

B. Alekseev

National Medical Research Radiological Center, Ministry of Health of Russia

Email: mosilkina@hse.ru
俄罗斯联邦, Moscow

参考

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2. Intravital micrographs of a culture of prostate cancer organoids in transmitted light, 2nd passage immediately after transplantation (a) and 14 days after transplantation, scale bar 400 μm (b), viability of organoids after treatment with docetaxel (red) and enzalutamide (blue) in concentration range from 0.625 to 100 µM (c) and the growth rate of organoids after treatment with docetaxel and enzalutamide at clinical concentrations (d), * means a statistical difference with the control (p < 0.05).

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