Karyotype and molecular genetic differentiation of a 24-chromosomal form of the gray hamster Nothocricetulus migratorius from the Tien Shan

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

The widespread Palaearctic rodent species gray hamster Nothocricetulus migratorius has a karyotype with a stable number of chromosomes 2n = 22 throughout the entire range of its habitat. We found gray hamsters with diploid number of chromosomes 2n = 24 locally distributed in the Qurama Ridge of the Tyan Shan. A new karyotype and analysis of G- and NORs-bands of differentially stained chromosome sets were described for the first time. The described karyotype differs from the 22-chromosomal karyotype of gray hamsters by the Y-chromosome morphology and the presence of an additional pair of heteromorphic small chromosomes. Molecular genetic analysis revealed genetic divergence of 24- and 22-chromosomal forms of N. migratorius, and the differences between them in mitochondrial markers are comparable, and in nuclear markers exceed the differences between C. barabensis (2n = 20) and C. psevdogriseus (2n = 24). The data obtained give grounds to discuss the taxonomic status of the 24-chromosomal form of gray hamsters from the Qurama Ridge and consider the differentiation of N. migratorius karyomorphs as a stage of chromosomal speciation.

全文:

受限制的访问

作者简介

O. Brandler

Koltzov Institute of Developmental Biology of Russian Academy of Sciences

编辑信件的主要联系方式.
Email: rusmarmot@yandex.ru
俄罗斯联邦, 119334, Moscow

A. Blekhman

Koltzov Institute of Developmental Biology of Russian Academy of Sciences

Email: rusmarmot@yandex.ru
俄罗斯联邦, 119334, Moscow

参考

  1. Громов И.М., Ербаева М.А. Млекопитающие фауны России и сопредельных территорий. Зайцеобразные и грызуны. СПб.: Наука. 1995. 522 с.
  2. Лебедев В.С. Cricetinae – Млекопитающие России. Систематико-географический справочник // Сб. тр. Зоол. музея МГУ. Т. 52 / под ред. Павлинов И.Я., Лисовский А.А. М.: Т-во научн. изданий КМК, 2012. С. 211–220.
  3. Lebedev V.S., Bannikova A.A., Neumann K. et al. Molecular phylogenetics and taxonomy of dwarf hamsters Cricetulus Milne-Edwards, 1867 (Cricetidae, Rodentia): Description of a new genus and reinstatement of another // Zootaxa. 2018. V. 4387. P. 331–349. https://doi.org/10.11646/zootaxa.4387.2.5
  4. Lebedev V., Poplavskaya N., Bannikova A. et al. Genetic differentiation in Cricetulus migratorius Pallas, 1773 (Rodentia, Cricetidae) // Mammalian Biology. 2018. V. 92. P. 115–119. https://doi.org/10.1016/j.mambio.2018.05.001
  5. Yerganian G., Papoyan S. Isomorphic sex chromosomes, autosomal heteromorphism, and telomeric associations in the grey hamster of Armenia, Cricetulus migratorius, Pall. // Hereditas. 1965. V. 52. № 3. P. 307–319. https://doi.org/10.1111/j.1601-5223.1965.tb01963.x
  6. Гайченко В.А. Хромосомный набор серого хомячка с территории Украины // Вестник зоологии. 1974. № 2. С. 79–80.
  7. Картавцева И.В. Внутривидовой хромосомный полиморфизм широкоареальных видов: краснохвостой песчанки и серого хомячка // Фенетика популяций. Саратов: 1985. С. 84–85.
  8. Брандлер О.В. Новая хромосомная форма серого хомячка Cricetulus migratorius Pallas, 1773 из Тянь-Шаня // VI съезд Териологического об-ва. Москва, 13–16 апреля 1999. Тез. докл. М.: 1999. С. 37.
  9. Ford C.E., Hamerton J.L. A colchicine, hypotonic citrate, squash sequence for mammalian chromosomes // Stain Technol. 1956. V. 31. № 6. P. 247–251.
  10. Seabright M. A rapid banding technique for human chromosomes // Lancet. 1971. V. 11. P. 971–972.
  11. Howell W.M., Black D.A. Controlled silver staining of nucleolus: A 1-step method // Experientia. 1980. V. 36. P. 1014–1015.
  12. Aljanabi S.M., Martinez I. Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques // Nucl. Ac. Res. 1997. V. 25 (22). https://doi.org/10.1093/nar/25.22.4692
  13. Jaarola M., Searle J.B. Phylogeography of field voles (Microtus agrestis) in Eurasia inferred from mitochondrial DNA sequences // Mol. Ecol. 2002. V. 11. P. 2613–2621. https://doi.org/10.1046/j.1365-294X.2002.01639.x
  14. Nakamura I., Ohnuma A., Ichihashi T. Mus musculus mitochondrial COI gene for cytochrome c oxidase subunit I, partial cds. 2008. http://www.ncbi.nlm.nih.gov/nuccore/193248428, дата обращения 30.11.2023 г.
  15. Lyons L.A., Laughlin T.F., Copeland N.G. et al. Comparative anchor tagged sequences (CATS) for integrative mapping of mammalian genomes // Nature Genetics. 1997. V. 15. № 1. P. 47–56.
  16. Steppan S.J., Storz B.L., Hoffmann R.S. Nuclear DNA phylogeny of the squirrels (Mammalia: Rodentia) and the evolution of arboreality from c-myc and RAG1 // Mol. Phylog. and Evol. 2004. V. 30. № 3. P. 703–719. https://doi.org/10.1016/S1055-7903(03)00204-5
  17. Edgar R.C. MUSCLE: multiple sequence alignment with high accuracy and high throughput // Nucl. Ac. Res. 2004. V. 32 № 5. P. 1792–1797. https://doi.org/10.1093/nar/gkh340
  18. Kumar S., Stecher G., Li M. et al. MEGA X: Molecular evolutionary enetics nalysis across computing platforms // Mol. Biol. and Evol. 2018. V. 35. P. 1547–1549. https://doi.org/10.1093/molbev/msy096
  19. Lavappa K.S. Chromosome banding patterns and idiogram of the Armenian hamster, Cricetulus migratorius // Cytologia. 1977. V. 42. № 1. P. 65–72.
  20. Radjabli S.I., Sablina O.V., Graphodatsky A.S. Selected karyotypes // Atlas of Mammalian Karyotypes / Eds O’Brien S.J., Nash W.G., Menninger J.C. Chichester: John Wiley, 2006. P. 209–221.
  21. Matthey R. Cytologie comparee des Cricetinae palearctiques et americains // Rev. Suisse Zool. 1961. V. 68. P. 41–61.
  22. Ахвердян М.Р. Особенности поведения половых хромосом в мейозе у серого хомячка (Cricetulus migratorius Pallas, 1770) // Генетика. 1993. Т. 29. № 6. С. 950–959.
  23. Kitano J., Peichel C.L. Turnover of sex chromosomes and speciation in fishes // Environ. Biol. of Fishes. 2012. V. 94. P. 549–558. https://doi.org/10.1007/s10641-011-9853-8
  24. Poplavskaya N., Bannikova A., Neumann K. et al. Phylogeographic structure in the chromosomally polymorphic rodent Cricetulus barabensis sensu lato (Mammalia, Cricetidae) // J. Zool. Syst. and Evol. Res. 2019. V. 57. № 3. P. 679–694. https://doi.org/10.1111/jzs.12251
  25. Romanenko S.A., Volobouev V.T., Perelman P.L. et al. Karyotype evolution and phylogenetic relationships of hamsters (Cricetidae, Muroidea, Rodentia) inferred from chromosomal painting and banding comparison // Chromosome Research. 2007. V. 15. P. 283–298. https://doi.org/10.1007/s10577-007-1124-3
  26. Орлов В.Н., Исхакова Э.Н. Таксономия надвида Cricetulus barabensis (Rodentia, Cricetidae) // Зоол. журн. 1975. Т. 54. В. 4. С. 597–604.
  27. Воронцов Н.Н. Развитие эволюционных идей в биологии. М.: И-во Отдел УНЦ ДО МГУ. Прогресс-Традиция, АБФ. 1999. 640 с.

补充文件

附件文件
动作
1. JATS XML
下载
2. Fig. 1. Karyotype of the male gray hamster N. migratorius 2n = 24. a – routine coloration, b – G-coloration, c – Ag-NOR-coloration. The arrows indicate the position of the YAP, the chromosome with the YAP on both shoulders is underlined. Cladograms of combined nucleotide sequences of hamsters based on the results of ML analysis: g – mtDNA (cytb and COI), d–nucleotide (BGN and C-myc). The numbers in the branch names correspond to the registration numbers of the samples in the IBR KTZ. The numbers at the nodes indicate bootstrap support indexes.

下载 (277KB)
索引源数据

版权所有 © Russian Academy of Sciences, 2024